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Journal of Bacteriology, December 2007, p. 9122-9125, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01451-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transposon Tn7 Directs Transposition into the Genome of Filamentous Bacteriophage M13 Using the Element-Encoded TnsE Protein

Jessica A. Finn,⁰ Adam R. Parks, and Joseph E. Peters^*

Department of Microbiology, Cornell University, Ithaca, New York

Received 7 September 2007/ Accepted 26 September 2007

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The bacterial transposon Tn7 has a pathway of transposition that preferentially targets conjugal plasmids. We propose that this same transposition pathway recognizes a structure or complex found during filamentous bacteriophage replication, likely by targeting negative-strand synthesis. The ability to insert into both plasmid and bacteriophage DNAs that are capable of cell-to-cell transfer would help explain the wide distribution of Tn7 relatives.

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Tn7 is a 14-kb bacterial transposon that only activates its transposition machinery when specific targets are found in the cell (reviewed in references 5 and 19). Tn7 can use two transposition pathways that recognize different types of target sites with distinct but overlapping sets of transposon-encoded proteins, TnsA, TnsB, TnsC, TnsD, and TnsE (TnsABCDE). The TnsABC proteins constitute the core transposition machinery that normally interacts with one of the two target site-selecting proteins, TnsD or TnsE, to carry out transposition. Transposition with the TnsABC+D proteins catalyzes Tn7 transposition into a single site found in Escherichia coli and a wide variety of other bacteria, called the attachment site (attTn7). TnsD is a sequence-specific DNA binding protein that recognizes a sequence in the C-terminal-encoding portion of glmS but directs transposition events into the attTn7 site in the terminator of glmS transcription. Transposition into the attTn7 site occurs at a single position and in a single orientation with no detectable negative effect on the host (8, 10, 11). Transposition via the TnsABC+E proteins does not recognize any particular DNA sequence but instead preferentially directs transposition into mobile plasmids, called conjugal plasmids, by recognizing an aspect of transfer-associated DNA replication (17, 18, 27). Presumably, the TnsE-mediated pathway would facilitate the dissemination of Tn7 to new hosts, while the TnsD-mediated pathway would provide a "safe haven" once in a new bacterial host.

The TnsABC+E transposition machinery recognizes a structure or complex found during active conjugal DNA replication. TnsE-mediated transposition events preferentially occur into the conjugal plasmid (>90%) in the cell, even though in these experiments the plasmid only comprises 1% of the DNA in the cell (17, 27). Nonconjugal plasmids are not preferred targets, and mobilizable plasmids are only targets when actively mobilized (27). Genetic results show that TnsE-mediated transposition occurs into conjugal plasmids in recipient cells, likely by recognizing a component of lagging-strand DNA synthesis during rolling-circle DNA replication. In the donor cell, leading-strand DNA synthesis displaces the strand that is then transferred to the recipient bacterium (7, 26). Because single-stranded DNA is introduced into the recipient cell in the 5'-to-3' direction, and because it is replicated as it enters the cell, DNA replication in the recipient cell must continually be reprimed in a discontinuous process. Tn7 displays an orientation bias in which the right end of the transposon is juxtaposed with the 3' end of the nascent lagging strand of conjugal plasmid replication. The same orientation bias with regard to the lagging strand occurs when TnsE directs transposition to targets found in chromosomal replication (17). We presume that something is special about lagging-strand DNA synthesis found during conjugation that preferentially attracts TnsE-mediated transposition.

Tn7 is remarkably prevalent in a variety of environments (16). While conjugal plasmids are abundant in environmental settings, additional targets beyond actively conjugating plasmids may exist that could facilitate the dissemination of Tn7 and its relatives. Bacteriophage P1 replication was previously shown to not be a TnsE-mediated transposition target (27); however, it is unknown whether the filamentous bacteriophage are a productive target.

TnsD-mediated transposition can occur into the genome of an M13 derivative carrying attTn7. One potential complication in determining if M13 is a transposition target is that the majority of the M13 genome encodes functions essential for bacteriophage growth. Therefore, we inserted 2.3 kb of exogenous DNA that would not be required for bacteriophage growth. We chose to clone the attTn7 region of the E. coli chromosome into M13mp18 using PCR because it included the specific site normally utilized by TnsD-mediated transposition that could serve as a positive control in our experiments (subsequently called M13-attTn7). When the M13-attTn7 bacteriophage was grown on a Tn7 donor strain containing a mini-Tn7 element with the TnsABC+D proteins provided in trans (JF55 pOX-Gen pCW4miniMu#76), bacteriophage conferring resistance to kanamycin (the transposon marker) were readily isolated (Tables 1 and 2). In this assay, bacteriophage were grown on the Tn7 donor strain for 2 hours to allow a 100-fold increase in bacteriophage. To determine the number of mini-Tn7-containing M13-attTn7 bacteriophage, 1.6 x 10⁹ bacteriophages were used to infect 5 x 10⁸ actively growing E. coli XL1-Blue cells as a reporter strain. M13 bacteriophage with mini-Tn7 was detected by the ability to confer kanamycin resistance. Transposition frequency was determined by dividing the number of CFU found on LB medium supplemented with kanamycin (50 µg/ml), tetracycline (20 µg/ml), and nalidixic acid (5 µg/ml) by the total number of infectious bacteriophage particles used to infect the host (tetracycline and nalidixic acid were used to counterselect against residual Tn7 donor cells in the bacteriophage lysate).

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TABLE 1. Bacterial strains used in this study

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TABLE 2. Plasmids and bacteriophages used in this study

Sequencing confirmed that transposition events were occurring at the single predicted position and orientation within the fragment and made the expected 5-bp target site duplication (5). The frequency of kanamycin-resistant bacteriophages was 4.8 x 10^–7 (kanamycin resistance-conferring bacteriophages per total bacteriophages) after 2 hours of growth. The frequency of kanamycin-resistant bacteriophages modestly increased at 4 and 6 hours, to 6.2 x 10^–7 and 7.1 x 10^–7, respectively. No kanamycin-resistant bacteriophage was found with a vector-only control (i.e., lacking transposition functions). This experiment indicated that M13 could tolerate the insertion of a mini-Tn7 element and that the attTn7 site was functional. This was consistent with a previous result with full-length Tn7; however, in the experiment presented here we can rule out any contribution of TnsE or other known transposon-encoded proteins in targeting an attTn7 site in M13 (21).

TnsE-mediated transposition occurs into the M13 genome. When M13-attTn7 was grown for 2 hours on a host that expressed the TnsABC+E proteins, we were also able to identify kanamycin resistance-conferring bacteriophage (Fig. 1). The wild-type TnsE protein allowed transposition at a frequency of about 3 x 10^–10 when expressed from a lac promoter (Fig. 1). We also examined the ability of a series of high-activity mutants that were isolated previously in a genetic screen (17). The mutant proteins are toxic when expressed at anything but very low levels and are therefore introduced on a vector with a very low copy number and expressed from the native tnsE promoter (17). Despite the lower expression levels, the increased activity mutants directed transposition into M13 at levels higher than the wild-type protein. In fact, we found that transposition could not be detected when wild-type TnsE was expressed at this level; wild-type TnsE needed to be expressed at higher levels to give any kanamycin resistance-conferring bacteriophage in this assay (Fig. 1 and data not shown).

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FIG. 1. Frequency of Tn7 transposition into the M13-attTn7 genome in E. coli in various genetic backgrounds. Wild-type TnsABC was expressed from pCW15 (wt) or a pCW15 derivative with the TnsCA225V mutation (mut) (24). pTA106 was included as a vector control (vector only). Wild-type or mutant derivatives of TnsE, TnsEA453V (A453V), TnsED523N (D523N), or the double mutant TnsEA453V+D523N (A453V D523N) were expressed from pJP103. Wild-type TnsE was expressed with pJP104 (wt*). M13-attTn7 bacteriophage containing mini-Tn7 in strains expressing wild-type TnsABC without TnsE, in the mutant TnsABCA225V, or with TnsABC with wild-type TnsE expressed from pJP103 was never identified (<3 x 10–11). A very low background of spontaneous nalidixic acid-resistant cells (10–11) was found in the assay where the F' Tn10 had mated into residual host strains from the XL1-Blue strain. These were easily identifiable because they contained all of the JF55 chromosomal and plasmid makers (i.e., resistance to rifampin, chloramphenicol, and ampicillin but sensitivity to gentamicin via loss of the pOX-Gen by replicon exclusion). Restriction analysis and DNA sequencing absolutely confirmed that the mini-Tn7 element was not contained on the M13-attTn7 plasmid. Error bars show the standard errors of the means (n = 9).

DNA sequencing revealed that TnsE-mediated transposition could occur throughout a large portion of the M13-attTn7 bacteriophage genome. This included 15 independent insertion events in the M13mp18 backbone and 10 independent insertions into the cloned fragment from the E. coli chromosome (Fig. 2). We assume that transposition events in the remainder of the M13 genome could not be detected because they would result in bacteriophage that could not be further propagated, a requirement for this assay. As expected, we were never able to isolate kanamycin resistance-conferring bacteriophage when the M13-attTn7 bacteriophage was grown with only the wild-type TnsABC; the core TnsABC machinery will not normally allow transposition without the TnsD or TnsE protein. We also tested whether random Tn7 transposition events could target the bacteriophage by using a mutant TnsABC^A225V core machinery. The TnsABC^A225V machine does not require TnsD or TnsE for activation in vivo or in vitro and appears to allow random transposition (2, 18). We were unable to recover any kanamycin-resistant M13-attTn7 bacteriophage using TnsABC^A225V.

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FIG. 2. Representation of the 9,505-bp M13-attTn7 bacteriophage genome and the position of the TnsE-meditated mini-Tn7 insertion events. Numbering follows the previously established convention for M13mp18 (28). The M13mp18 sequence extends from bp 8529 to 9505/1 to 6266. The M13 origin (ori; bp 5487 to 5867), 'glmS (bp 6267 to 7371), pstS' (bp 7708 to 8529), and the position of TnsD-mediated insertion events (attTn7) are indicated. Independent transposition events are indicated by arrows either inside (left-to-right insertion events) or outside (right-to-left insertion events) the circle. The positions of mini-Tn7 insertion events are indicated in parentheses along with the strain background according to the following letter code: A, pCW15 (TnsABC) pJP104 (TnsEwt); B, pCW15 pJP103 (TnsEA453V); C, pCW15 pJP103 (TnsED523N); D, pCW15 pJP103 (TnsEA453V+D523N). The position and orientation of individual transposition events were determined by isolating the replicative (double-stranded) form of the bacteriophage and sequencing from the left end of the element using a primer complementary to this end. To confirm that the process of transposition was responsible for relocating the element into the bacteriophage, we sequenced a subset of the insertions from both ends and in all cases identified a 5-bp duplication that is indicative of Tn7 transposition. The asterisk indicates where identical insertions were found in this experiment that are likely siblings.

The TnsE-mediated transposition events occurred at many different positions in M13, consistent with previous results with the TnsE pathway of transposition; TnsE-mediated transposition is not specific for any particular DNA sequence (Fig. 2). However, the TnsE-mediated insertions occurred with a striking right-to-left orientation bias that was reminiscent of a bias with DNA replication identified in conjugal plasmids and in the chromosome (17). By comparing the Tn7 orientation bias found in the chromosome and conjugal plasmids with these new results with M13, we can surmise which process of the M13 life cycle is targeted.

Previous results suggest that recessed 3' ends are recognized by TnsE, which targets Tn7 into the adjacent duplex DNA in a specific right-to-left orientation. The observations presented here are consistent with this hypothesis and indicate that negative-strand DNA synthesis attracts TnsE-mediated transposition events into the M13 genome (Fig. 3) (17, 19). Filamentous bacteriophages are packaged as single-stranded DNA circles that must be made double-stranded when introduced into a new host (14). Negative-strand DNA synthesis is required to produce the complementary DNA strand after bacteriophage infection. Negative-strand synthesis is further required for a short window of time (20 min) to make additional copies of the replicative form of the bacteriophage genome. After a sufficient number of the replicative forms of the M13 genome have accumulated in the cell, positive-strand replication generates single-stranded, circular copies of the genome, which are coated with a bacteriophage-encoded single-strand DNA binding protein, gp5, before extrusion through the cell envelope.

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FIG. 3. Orientation of TnsABC+E transposition events in a replicating chromosome, F plasmid (pOX-Gen), and M13. TnsE-mediated transposition events occur in a single orientation with lagging-strand DNA replication in the chromosome and the F plasmid derivative pOX-Gen (19). The orientation bias found in this work suggests that negative-strand DNA synthesis attracts Tn7 insertion events in the TnsABC+E pathway (see text for details). Rectangles represent Tn7, with the right (R) and left (L) ends of the element indicated. Arrowheads show the 3' ends of the DNA strands. The negative and positive strands of M13 are indicated. The F plasmid TraI protein is shown with a hatched circle bound to the 5' end of the conjugating plasmid. On the chromosome, the origins of chromosomal DNA replication (oriC) and replication by lagging-strand DNA replication are indicated.

Frequency and targeting of transposition. High-frequency transposition with a random transposition pathway catalyzed with the mutant TnsABC^A225V machinery did not give detectable transposition into the M13-attTn7 vector (Fig. 1). Transposition with the core machinery with this TnsC^A225V mutant allows transposition into the chromosome that is approximately 10-fold higher than levels found with TnsABC+E (24). The lack of observable transposition with the TnsABC^A225V machinery into the M13-attTn7 vector, under conditions in which we do see transposition with TnsABC+E, suggests that the ability to target the M13 genome is specific to the TnsE pathway. This result is consistent with previous results with conjugal DNA replication (24, 27).

The low frequency of transposition into the bacteriophage genome is likely due to the limited availability of duplex DNA during the M13 life cycle; duplex DNA is required for a DNA molecule to act as a transposition target, because only the 3' ends of the element are joined to a target DNA (6). The short amount of time that the filamentous bacteriophage normally spends as duplex DNA may partially protect this type of bacteriophage from being targets for transposition and likely explains why we were unable to detect transposition events into the M13-attTn7 vector with the random TnsABC^A225V pathway of transposition (i.e., M13 may need to be actively targeted). We found that TnsABC^A225V will target the double-stranded, replicative-form M13-attTn7 vector in vitro when it is the only target DNA in the reaction mixture, indicating that the vector can be a target for transposition with the mutant core machinery (A. Parks and J. E. Peters, unpublished observation). TnsABC+E transposition appears to be attracted to regions of discontinuous negative-strand synthesis, even though this type of replication only makes up a brief portion of the filamentous bacteriophage life cycle. Consistent with previous results, the high-activity TnsE mutants allowed for increased levels of transposition and occurred with the same orientation bias as transposition events with wild-type TnsE (17).

Tn7 has two pathways of transposition that facilitate the dissemination and establishment of the element within a bacterial genome while minimizing the impact on the host bacterium. Here we have described how Tn7 targets a filamentous bacteriophage genome, suggesting an expanded repertoire of vectors capable of transferring Tn7 from host to host.

 
We thank Nancy Craig (Johns Hopkins Medical School and Howard Hughes Medical Institute) and the E. coli Genetic Stock Center for providing bacterial strains and plasmids. We thank the members of the Peters lab for comments on the manuscript.

This work was funded by a grant from the National Science Foundation (MCB-0315316).

 
* Corresponding author. Mailing address: Department of Microbiology, Cornell University, 175a Wing Hall, Ithaca NY 14853. Phone: (607) 255-2271. Fax: (607) 255-3904. E-mail: jep48{at}cornell.edu

Published ahead of print on 5 October 2007.

Present address: NYU Medical Center, 550 First Ave., New York, NY 10016.

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Journal of Bacteriology, December 2007, p. 9122-9125, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01451-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Finn, J. A. Articles by Peters, J. E. Search for Related Content PubMed PubMed Citation Articles by Finn, J. A. Articles by Peters, J. E.