Characterization of the Saframycin A Gene Cluster from Streptomyces lavendulae NRRL 11002 Revealing a Nonribosomal Peptide Synthetase System for Assembling the Unusual Tetrapeptidyl Skeleton in an Iterative Manner
J. Bacteriol. Li et al.
190: 251
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
Table S1, deduced function of ORFs beyond the SFM-A biosynthetic gene cluster boundary; Table S2, comparison of the eight specificity-conferring codes of A domains; Fig. S1, structures of SFMs; supplemental methods; Fig. S2, localization and restriction map of the cloned DNA region from S. lavendulae NRRL 11002; Fig. S3, HPLC analysis of SFM-A production; Fig. S4 and S5, MS spectra; Scheme S1 to S4, synthesis of OmTyr, serine iodide, 3mOmTyr, and 3h5mOmTyr
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