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Journal of Bacteriology, July 2008, p. 4749-4753, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.01988-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evidence for an Elongated Dimeric Structure of Heparin-Binding Hemagglutinin from Mycobacterium tuberculosis{triangledown}

Carla Esposito,1 Maxim V. Pethoukov,2 Dmitri I. Svergun,2 Alessia Ruggiero,1 Carlo Pedone,1,3 Emilia Pedone,1* and Rita Berisio1*

Istituto di Biostrutture e Bioimmagini, C.N.R., I-80134, Napoli, Italy,1 European Molecular Biology Lab, Hamburg, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany,2 Dipartimento delle Scienze Biologiche, Sezione di Biostrutture, Università degli Studi di Napoli Federico II, I-80134, Napoli, Italy3

Received 21 December 2007/ Accepted 13 April 2008


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ABSTRACT
 
Heparin-binding hemagglutinin (HBHA) is a virulence factor of tuberculosis which is responsible for extrapulmonary dissemination of this disease. A thorough biochemical characterization of HBHA has provided experimental evidence of a coiled-coil nature of HBHA. These data, together with the low-resolution structures of a full-length form and a truncated form of HBHA obtained by small-angle X-ray scattering, have unambiguously indicated that HBHA has a dimeric structure with an elongated shape.


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TEXT
 
Mycobacterium tuberculosis is one of the most devastating human microbial pathogens. It invades and multiplies in both phagocytes and epithelial cells (15). Adherence to host cells is mediated in bacteria by a plethora of fibrous systems, such as pili, fimbriae, and capsules (2, 23, 29). In M. tuberculosis, adherence to epithelial cells is mediated predominantly by the heparin-binding hemagglutinin adhesin (HBHA) (14, 18). This protein binds to heparan sulfate proteoglycans on the surface of epithelial cells and is therefore responsible for extrapulmonary dissemination of tuberculosis (19, 20). Binding to target epithelial cells involves the C-terminal lysine-rich domain of the protein, which is exposed at the mycobacterial cell surface (16). Like other bacterial adhesins, HBHA also expresses hemagglutination activity. This process has been proposed to be due to interactions between HBHA molecules on the surface of mycobacteria (4, 16). In particular, hemagglutination has been correlated with the ability of the N-terminal region of HBHA to form multimers (4, 28). However, despite the interest in HBHA both as a potential antigen against tuberculosis (13, 17) and as a diagnostic tool (6), no structural data are so far available, nor has detailed information on the protein oligomerization state yet been provided. We here present a biochemical characterization and the low-resolution structure determined by small-angle X-ray scattering (SAXS) of both the full-length HBHA and a truncated form, here denoted HBHA{Delta}C, which lacks the C-terminal heparin-binding domain (residues 161 to 199). The results presented here provide clues to the molecular bases of bacterial agglutination processes.

Methods. (i) Cloning, expression, and purification of proteins. The genes coding for HBHA and HBHA{Delta}C (lacking residues 161 to 199) were amplified from the M. tuberculosis (strain H37Rv) genome by PCR and introduced into the pET-28a(+) (Novagen) and pETM-11 expression vectors, respectively. Sequenced clones were transformed in the Escherichia coli BL2(DE3) strain. Cells were grown to an optical density at 600 nm of approximately 0.5 in LB medium supplemented with kanamycin (50 µg/ml) at 37°C and were induced by 1 mM isopropyl-β-D-thiogalactoside (IPTG) for 5 h.

HBHA was purified on a heparin Hi-trap column (GE Healthcare) (10 mM Na phosphate, pH 7.0). The protein was eluted with a 0 to 1 M NaCl linear gradient. The proteic sample was further purified by size exclusion chromatography on Superdex 200 (GE Healthcare) (50 mM Tris-HCl,100 mM NaCl, pH 8.0). HBHA{Delta}C protein was purified by Ni-nitrilotriacetic acid affinity chromatography (GE Healthcare) (equilibrated with 10 mM imidazole, 300 mM NaCl, and 50 mM Tris-HCl, pH 8.0) and eluted with a linear gradient of imidazole (10 to 300 mM). Following histidine tag removal, the protein was further purified by size exclusion chromatography on Superdex 200 (GE Healthcare).

(ii) Cross-linking experiments. Cross-linking analysis of HBHA{Delta}C was performed by incubating 1.2 mg ml–1 HBHA{Delta}C in 20 mM HEPES buffer (pH 7.5), with either glutaraldehyde (0.3 to 2.3%) for 5 min at 37°C or bis-sulfosuccinnimidyl-suberate (BS3) (protein/BS3 ratios of 1:10 to 1:30) for 30 min at 25°C. Samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

(iii) CD experiments. Circular dichroism (CD) spectra were recorded in 10 mM Na phosphate buffer (pH 7.0) in the wavelength range of 185 to 260 nm using a Jasco J-810 spectropolarimeter equipped with a Peltier thermostatic cell holder. Heat- and urea-induced denaturations were investigated by recording the CD signal at 222 nm. The final concentration of urea was in the range of 0.0 to 5.5 M, and each sample was incubated overnight at 293 K.

(iv) SAXS experiments. Synchrotron SAXS data were collected on the X33 beamline (24) at EMBL, DESY, Hamburg. Scattering patterns of truncated and full-length HBHA were measured at several solute concentrations ranging from 2.0 to 7.7 mg/ml, and the range of momentum transfer 0.6 < s < 5.0 nm–1 [s = 4{pi} sin({theta})/{lambda}, where 2{theta} is the scattering angle and {lambda} = 0.15 nm is the X-ray wavelength] was covered. Data were processed using standard procedures by PRIMUS (10). The molecular masses of the solutes were estimated based on the excluded (Porod) volume, Vp (22). For proteins, Porod (i.e., hydrated) volumes in nm3 are approximately two to three times larger than those expected given the molecular masses in kDa. Low-resolution models of HBHA were generated by two alternative ab initio approaches. The program GASBOR (27) represents a protein by an assembly of dummy residues and uses simulated annealing to build a locally "chain-compatible" dummy residue model inside a sphere with the diameter of the maximum particle size, Dmax. The multiphase bead modeling program MONSA (21, 26) represents the particle as a collection of densely packed beads inside the same spherical search volume. To describe the structure of HBHA, each bead can be assigned either to the solvent (index = 0) or to the HBHA{Delta}C part or the C terminus (index = 1 and 2, respectively). Starting from random phase indices, simulated annealing was employed to search for a model which simultaneously fits the data for HBHA{Delta}C and the full-length protein. The results of multiple reconstructions were averaged by the package DAMAVER (9) and SUPCOMB (11). The latter program aligns two arbitrary low- or high-resolution models by minimizing a dissimilarity measure, NSD, which is computed from the distances between two bead or atomic models as defined in reference 11. Generally, NSD values close to unity indicate that the two models are similar. The program DAMAVER selects the most typical model (i.e., that having the lowest average NSD with all the other models of the set of superimposed structures).

HBHA secondary structure. In order to experimentally assess the secondary structure content of HBHA, we performed CD experiments with both HBHA and HBHA{Delta}C. The spectra of the two proteins are quite similar and are characterized by two negative broad peaks at about 208 and 220 nm (Fig. 1A). These spectra, with an {alpha}-helix band intensity at 208 nm stronger than that at 222 nm, are typical of proteins with a coiled-coil structure (1, 8, 12, 25). To validate this result, we performed chemical denaturation, using urea as a denaturating agent, of HBHA{Delta}C. The urea denaturation profile of HBHA{Delta}C is characteristic of a two-state helix-coil transition with a single infection point, Cm, centered at 1.8 M urea (Fig. 1B). This denaturation profile, with a peculiarly low urea Cm, has often been encountered in denaturation studies of coiled-coil systems (8). Thermal denaturation curves, recorded at 222 nm, show that denaturation occurs in one step (data not shown). Furthermore, folding of both proteins is fully reversible (Fig. 1A). This is consistent with the observation that in oligomeric structures, coiled coils often form in a single-step reaction, in which association and folding of peptide chains are tightly coupled (7). A coiled-coil propensity of HBHA is also predicted by sequence analysis (4, 27). Indeed, the HBHA sequence reveals the typical pattern of left-handed coiled coils, with a periodicity of seven (heptad repeats, abcdefg). As expected for left-handed coiled coils, residues in positions a and d are mainly hydrophobic, whereas those in positions e and g are mainly charged (Fig. 2). The highest propensity to form a coiled coil was calculated (using the program PCOILS [5]) for the region from residue 24 to 69, followed by the region from residue 87 to 107 (Fig. 2). The coiled-coil region from residue 24 to 69 was also predicted, using the program MULTICOIL (31), to have a high propensity to form two-stranded coiled coils (Fig. 2).


Figure 1
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  		FIG. 1. (A) CD curves of HBHA and HBHA{Delta}C. CD curves after refolding are also shown. Melting temperatures are 37°C and 47°C for HBHA and HBHA{Delta}C, respectively. (B) Urea-induced denaturation of HBHA{Delta}C; experimental values are fitted with a sigmoidal curve. (C) SDS-PAGE analysis of HBHA{Delta}C after cross-linking with glutaraldehyde. Lane 1 contains 50 µg of HBHA{Delta}C as a control; lanes 2 to 6 contain 50 µg, 60 µg, 75 µg, 90 µg, and 100 µg of HBHA{Delta}C, respectively; lane 7 contains molecular mass markers (66, 45, 36, 29, 24, 20, and 14.2 kDa). (D) SDS-PAGE analysis of HBHA{Delta}C after cross-linking with BS3. Lanes 1 to 3 contain 30 µg HBHA{Delta}C and protein/BS3 ratios of 1:10, 1:20, 1:30, respectively.


Figure 2
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  		FIG. 2. (A) Heptad repeats found in the HBHA sequence with the highest coiled-coil propensity, as predicted by the program PCOILS (5). Numbers on the left are amino acid residues. (B) Probability of residues forming either two-stranded (solid line) or three-stranded (dashed line) coiled coils, as predicted by the program MULTICOIL (31).

HBHA oligomerization state. The evidenced coiled-coil nature of HBHA accords well with the hemagglutination function of HBHA, since coiled coils are folding motifs which guide oligomerization in a variety of systems (1, 12, 30). The oligomerization state of HBHA is not clear to date, as atomic force microscopy studies have indicated HBHA to be either dimeric or multimeric (28). Gel filtration profiles of freshly purified proteins showed the existence of species with molecular masses of 88 kDa for HBHA and 66 kDa for HBHA{Delta}C. These estimates are compatible with a tetrameric arrangement of the proteins. However, since estimates of molecular masses by gel filtration depend on the protein shape, we decided to use two further approaches. First, we performed cross-linking experiments, followed by gel electrophoresis in denaturant conditions (15% SDS-PAGE), carried out using both glutaraldehyde and BS3 as cross-linking agents. These studies consistently indicated a molecular mass for HBHA{Delta}C of 34 kDa, which corresponds to a dimer (Fig. 1C and D). Indeed, traces of mass with a molecular weight compatible with a tetrameric assembly (about 70 kDa) were detected only when a large excess of BS3 was used (Fig. 1D). The gel filtration profiles of cross-linked species were superposable with that of HBHA{Delta}C, indicating that no unspecific cross-link had occurred. Cross-linking experiments were also performed with full-length HBHA and showed the existence of dimers, tetramers, and higher-order oligomers (not shown). The high-order oligomers were judged to be artifacts attributed to the lysine-rich C terminus (14 residues), which provides many cross-linking sites.

In order to further validate this result and to understand its inconsistency with results from gel filtration, we performed SAXS experiments with both HBHA and HBHA{Delta}C. The scattering patterns from HBHA and HBHA{Delta}C are presented in Fig. 3, and the structural parameters computed from the SAXS data are given in Table 1. The experimental Rg and Dmax values indicate that the particles are elongated, and the computed volumes are compatible with a dimeric organization of both constructs in solution. The increase of both the Rg and Dmax of the full-length protein compared to HBHA{Delta}C suggests a peripheral location of the C terminus of HBHA.


Figure 3
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  		FIG. 3. SAXS modeling. (Top) Typical ab initio models of HBHA{Delta}C (1) and full-length HBHA (2) generated using GASBOR (left and middle, respectively) and MONSA (right). In the MONSA representation, beads corresponding to the C termini are colored in green whereas the rest of the molecule is colored in blue. (Bottom) SAXS profiles from HBHA{Delta}C (1) and the full-length protein (2). The experimental data are denoted by dots, and fits from dummy residue and bead models are given as solid red lines and blue triangles, respectively. The curves are in logarithmic scale for better visualization.


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  		TABLE 1. Overall SAXS parametersa

Elongated structure of HBHA dimer determined by SAXS. As an additional check of the HBHA oligomeric state, several ab initio dummy residue reconstructions of both HBHA and HBHA{Delta}C were performed by GASBOR (27), assuming monomeric, dimeric, trimeric, and tetrameric organizations. For dimers and tetramers the models were built assuming no symmetry and also with P2 and P222 (tetramers only) symmetry constraints. In all cases, the best agreement with the experimental data was achieved when dimers were assumed (Table 1). GASBOR modeling attempts with dimeric HBHA and HBHA{Delta}C (both assuming P2 symmetry and without symmetry restrictions) yielded reproducible models (with an average NSD between the reconstructions of ~1.5) whereby the restored shapes of HBHA{Delta}C could be well accommodated within those for HBHA. This allowed us to assume the same conformation of the fragment from residue 1 to 160 in the truncated and full-length proteins and to fit the two data sets simultaneously by MONSA using twofold symmetry. A typical MONSA model (Fig. 3) agrees well with GASBOR dimeric models and neatly fits the experimental data (Table 1). All reconstructed models consistently show that HBHA displays an elongated shape with the C termini located at the outer ends (Fig. 3).

Conclusions. We present here the first structural characterization of HBHA from M. tuberculosis. We have cloned, expressed, and purified both a full-length form and a truncated form of HBHA and applied various biophysical techniques to study its molecular properties and oligomerization, a key event for bacterial hemagglutination. CD studies have provided experimental evidence that HBHA presents a coiled-coil domain (Fig. 1). In addition, SAXS and cross-linking experiments have unambiguously shown that HBHA exists in solution as a dimer with an elongated shape (Fig. 3). SAXS results also indicate a peripheral location of the C-terminal positively charged arms of HBHA (Fig. 3). The protrusion of C termini toward the same side of the protein is consistent with the role attributed to the HBHA C terminus in driving the binding of bacteria by establishing electrostatic interactions with epithelial heparan sulfates (18). Overall, our data suggest that, like for other known systems (1, 3), the coiled-coil nature of the N-terminal region (residues 25 to 135) of HBHA is responsible for dimerization, which may be regarded as a process that leads to enhanced affinity to epithelial cells and improves cell adhesion.


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ACKNOWLEDGMENTS
 
This work was supported by the Italian Human ProteomeNet. M.V.P. and D.I.S. acknowledge support from the EU design study SAXIER (contract RIDS no. 011934).

We thank G. D'Auria for discussion.


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FOOTNOTES
 
* Corresponding author. Mailing address: Istituto di Biostrutture e Bioimmagini, C.N.R., I-80134, Napoli, Italy. Phone for R. Berisio: 39 081 2534507. Fax: 39 081 2536642. E-mail: rita.berisio{at}unina.it. Phone for E. Pedone: 39 081 2534521. Fax: 39 081 2536642. E-mail: empedone{at}unina.it Back

{triangledown} Published ahead of print on 25 April 2008. Back


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Journal of Bacteriology, July 2008, p. 4749-4753, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.01988-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Verbelen, C., Dupres, V., Raze, D., Bompard, C., Locht, C., Dufrene, Y. F. (2008). Interaction of the Mycobacterial Heparin-Binding Hemagglutinin with Actin, as Evidenced by Single-Molecule Force Spectroscopy. J. Bacteriol. 190: 7614-7620 [Abstract] [Full Text]  

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