Cel9D, an Atypical 1,4-β-D-Glucan Glucohydrolase from Fibrobacter succinogenes: Characteristics, Catalytic Residues, and Synergistic Interactions with Other Cellulases
J. Bacteriol. Qi et al.
190: 1976
Supplemental material
Files in this Data Supplement:
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Fig. S1, separation of whole-cell extract from F. succinogenes; Fig. S2, zymogram of fractions containing high glucanase activity; Fig. S3, amino acid sequence of Cel9D; Fig. S4, relationships between F. succinogenes and other representative family 9 glycoside hydrolases; Fig. S5, SDS-PAGE analysis of Cel45C, Cel9D, Cel9D-ΔS168, and Cel9D-S168A; Fig. S6, hydrolysis of pNPC and ASC by Cel9D; Fig. S7, partial 1H NMR spectra; Table S1, predicted subsite affinities of Cel9D for cellooligosaccharide glucose residues
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