Files in this Data Supplement:

• Supplemental File 1 - Legends to Fig. S1 through S6
  Zipped PDF file, 96K.
• Supplemental File 2 - Table S1, differences in expression between CF39s and CF39wt
  Zipped PDF file, 105K.
• Supplemental File 3 - Fig. S1, MJK8 has an in-frame deletion of wspA
  Zipped TIFF file, 978K.
• Supplemental File 4 - Fig. S2, liquid cultures of MJK8 and CF39s
  Zipped TIFF file, 1.6MB.
• Supplemental File 5 - Fig. S3, only mutations in both the pel and psl loci eliminate the RSCV phenotypes of MJK8
  Zipped TIFF file, 871K.
• Supplemental File 6 - Fig. S4, 2D-TLC assays showing c-di-GMP levels of cupA3 mutant strains
  Zipped TIFF file, 315K.
• Supplemental File 7 - Fig. S5, MJK8 harboring mutations in the pel and psl loci fails to autoaggregate but produces elevated c-di-GMP
  Zipped TIFF file, 839K.
• Supplemental File 8 - Fig. S6, Biolog assay growth plots of PAO1 and mutants
  Zipped TIFF file, 9.2MB.