Files in this Data Supplement:

• Supplemental file 1 - Supplemental methods
  Word file, 37K.
• Supplemental file 2 - Table S1, primers
  Word file, 48K.
• Supplemental file 3 - Table S2, twitching assay results
  Word file, 27K.
• Supplemental file 4 - Fig. S1, elution of CbpA from cAMP-conjugated agarose
  Word file, 237K.
• Supplemental file 5 - Fig. S2, growth curve of PAO1 and PAO1ΔcbpA
  Word file, 85K.
• Supplemental file 6 - Fig. S3, biofilm assay
  Word file, 57K.
• Supplemental file 7 - Fig. S4, protease secretion assay
  Word file, 49K.
• Supplemental file 8 - Fig. S5, flagellum-dependent swimming motility assay
  Word file, 45K.
• Supplemental file 9 - Fig. S6, ExoT secretion assay
  Word file, 98K.
• Supplemental file 10 - Fig. S7, LDH assays
  Word file, 57K.
• Supplemental file 11 - Fig. S8, CbpA is not required for virulence in a mouse model of acute pneumonia
  Word file, 101K.
• Supplemental file 12 - Fig. S9, protein expression profiles of PAO1 and mutant strains
  Word file, 2.3MB.
• Supplemental file 13 - Fig. S10, predicted structural model of CbpA
  Word file, 618K.