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Journal of Bacteriology, February 2009, p. 1106-1110, Vol. 191, No. 3
0021-9193/09/$08.00+0     doi:10.1128/JB.00599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genome-Wide Identification of H-NS-Controlled, Temperature-Regulated Genes in Escherichia coli K-12{triangledown} ,{dagger}

Christine A. White-Ziegler* and Talya R. Davis{ddagger}

Department of Biological Sciences and Program in Biochemistry, Smith College, Northampton, Massachusetts

Received 30 April 2008/ Accepted 10 November 2008


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ABSTRACT
 
DNA microarrays demonstrate that H-NS controls 69% of the temperature regulated genes in Escherichia coli K-12. H-NS is shown to be a common regulator of multiple iron and other nutrient acquisition systems preferentially expressed at 37°C and of general stress response, biofilm formation, and cold shock genes highly expressed at 23°C.


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TEXT
 
Temperature is one of the many signals bacteria use as a cue for modulating gene expression. Genome-wide studies have provided evidence that human body temperature has a broad role in regulating gene expression patterns that facilitate effective host colonization (3, 11, 18, 21, 26, 28, 31, 33, 39), while low growth temperatures influence the expression of genes required for adaptation to vector hosts, aquatic environments, or biomedically relevant ambient room settings (3, 11, 18, 20, 21, 26, 28, 31, 33, 34, 40).

The histone-like nucleoid structuring (H-NS) protein, conserved among gram-negative bacteria, regulates the transcription of many environmentally responsive genes, implicating this regulator in bacterial adaptation to changing conditions, including temperature (reviewed in references 4 and 24). In Escherichia coli, temperature regulation and H-NS have primarily been studied in the control of specific operons related to virulence, including fimbriae, toxins, and pathogenicity island-associated genes. (1, 7, 8, 10, 14, 15, 19, 22, 25, 35-37, 41, 42).

In this study, the genome-wide role of H-NS in controlling temperature-regulated genes was investigated in E. coli K-12. Gene expression ratios of an hns651 mutant strain grown at 37 and 23°C were determined and subsequently compared to those ratios obtained with the wild-type strain in previous microarrray studies (39, 40) to identify H-NS-mediated changes as a function of temperature. The strain used (DL1947) contains an insertion in hns that abrogates expression of the H-NS protein but is otherwise identical to the wild-type strain (DL1504) (9, 37). The hns651 mutant was grown as described previously (39) at 37 and 23°C in M9 glycerol medium with aeration, and RNA was harvested in exponential phase at 9 to 11 generations of growth after inoculation, thus reflecting genes whose expression is differentially maintained over long-term growth at 37 and 23°C. cDNAs were cohybridized to microarray slides containing oligonucleotides representing all of the genes of E. coli K-12; H-NS-controlled genes are those in which the ratio of gene expression measured at the two temperatures in the hns651 mutant differed from that measured in the wild-type strain. This change in the expression ratio indicates a role for H-NS in regulation by altering transcription at either one or both temperatures. While comparison of these microarray data sets obtained at different time points did not offer gene expression ratios (mutant/wild type) at each temperature, it allowed the sensitive detection of thermally H-NS-regulated genes.

H-NS controls more than two-thirds of the temperature-regulated genes in E. coli K-12 but also many nonthermoregulated genes. Of the 122 thermoregulated genes with increased expression at 37°C compared to 23°C, 73 were identified as being H-NS controlled (Table 1; see Table S1 in the supplemental material). For 60 of these genes, the absence of H-NS reduced or eliminated a thermoregulatory response whereas 13 genes showed a statistically significant expansion of differential expression between 37 and 23°C. Several genes identified by this strategy (srlAD, cysPWU, garLPR, fes, and cirA) were previously shown to be thermoregulated and H-NS controlled by quantitative reverse transcription-PCR (qRT-PCR) (39), supporting the validity of the approach.


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  		TABLE 1. Characterization of H-NS regulated genesa

Of the 297 genes more highly expressed at 23°C in wild-type bacteria, 215 are regulated by H-NS. Of these genes, 179 showed a reduced or total loss of a thermoregulatory response between 37 and 23°C in the hns651 mutant whereas 36 demonstrated a statistically significant expanded thermoregulatory differential (Table 1; see Table S2 in the supplemental material).

For genes that did not show a thermoregulatory response in the wild-type strain, a large number demonstrated a statistically significant difference in expression between 37 and 23°C in the hns651 mutant. Increased expression in the hns651 mutant at 37°C was observed for 308 genes (Table 1; see Table S3 in the supplemental material), whereas 264 showed increased expression at 23°C (Table 1; see Table S4 in the supplemental material), indicating that H-NS contributes to the regulation of many genes that are unaffected by growth temperature.

Comparison to other genome-wide analyses reveals the direct targets of H-NS binding and that H-NS regulates many genes common to both pathogenic and nonpathogenic E. coli strains. Oshima et al. characterized approximately 250 H-NS binding sites within the E. coli K-12 genome by chromatin immunoprecipitation (ChIP)-chip analyses (27). Binding sites either 5' upstream or within their coding sequences mapped to genes we identified as being H-NS regulated, including 19% of the genes (14 genes) more highly expressed at 37°C (see Table S1 in the supplemental material) and 33% of the genes (70 genes) more highly expressed at 23°C (see Table S2 in the supplemental material), suggesting that the transcriptional effects at these promoters are due to direct binding of the H-NS protein. Among those genes that are not temperature regulated but demonstrate H-NS regulation, there was also a significant overlap with the H-NS binding site database. Approximately 10% of the genes (34 genes) more highly expressed at 37°C (see Table S3 in the supplemental material) and 45% of the genes (120 genes) more highly expressed at 23°C (see Table S4 in the supplemental material) in the hns651 mutant have H-NS binding sites associated with the operons that contain them. Interestingly, there are similar numbers of genes that are direct targets of H-NS, regardless of whether or not they are thermoregulated.

In comparison to other genome-wide studies assessing H-NS control of transcription at a single temperature, 157 H-NS-regulated genes identified in our study overlap those in uropathogenic strain 536 (23) and 47 genes in E. coli K-12 (13) (see Tables S1 to S4 in the supplemental material), encompassing both thermoregulated and nonthermoregulated genes.

H-NS regulates 60% of the genes more highly expressed at 37°C and is a common regulator of multiple iron uptake systems in E. coli. Our previous studies demonstrated that the mammalian host temperature (37°C) serves to increase and maintain 122 genes at a higher steady-state level of expression compared to 23°C (39), and the results presented here show that H-NS contributes to the regulation of 60% of these genes. The majority of these genes are involved in nutrient uptake—amino acid transport and metabolism (16), carbohydrate transport and metabolism (14), and inorganic ion transport and metabolism (11)—and their higher expression at 37°C may be particularly beneficial to host colonization.

Of the genes with increased expression at 37°C, 10 iron utilization genes in seven different operons are temperature regulated and controlled by H-NS. To corroborate the microarray results and demonstrate how H-NS specifically contributes to gene expression, relative mRNA levels at 37 and 23°C in the wild-type and hns651 mutant strains were analyzed by qRT-PCR as previously described (39) (Table 2). Representative genes within the ferric enterobactin (fep), ferric citrate (fec), ferrichrome (fhu), and ferrous (feo) systems were investigated to determine if H-NS control is broadly applicable to iron uptake. For genes in the fep, fec, and fhu systems, expression of the iron utilization genes in the wild-type strain is reduced at 23°C compared to that at 37°C, confirming temperature as a common regulatory cue for these genes (Table 2). In the hns651 mutant, the expression of all of these genes is statistically significantly decreased at 37°C compared to that in the wild-type strain, indicating a positive role for H-NS. At 23°C, the effect of H-NS on expression is variable in these systems, with some being unaltered by the hns651 mutation (fecA, fecI, fhuE) whereas others are reduced (fepC, fepD, fhuA) in comparison to the wild-type strain, indicating a positive regulatory role. Previous studies in our laboratory show a similar trend for two other iron acquisition genes, cirA and fes (39).


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  		TABLE 2. Iron utilization gene mRNA levels at 37 and 23°C in wild-type and hns651 mutant strainsa

In contrast to the other iron uptake systems, the feoA gene appears not to be temperature regulated and reveals equivalent expression levels at both 37 and 23°C (Table 2). The introduction of the hns651 mutation led to significantly increased expression at both 37 and 23°C in comparison to that in the wild-type strain (Table 2). It is interesting that H-NS binding sites are associated with the feoAB operon based on ChIP-chip analyses (27), correlating to the only operon we tested at which H-NS acts purely as a repressor of transcription. With the exception of fepE, no binding sites for H-NS were found to be associated with any of the iron utilization genes within the fep/ent, fhu, or fec system, arguing that the effect on gene expression in these operons by H-NS is likely indirect.

Fur (ferric uptake regulator) is an obvious candidate for an intermediate regulator targeted by H-NS. Transcription of the genes in these systems is responsive to the iron concentration, showing high expression under iron-depleted conditions but repressed by the transcriptional regulator Fur when iron is present (reviewed in references 6 and 12). We hypothesized that H-NS might repress fur transcription, resulting in increased transcription of this repressor in an hns651 mutant strain. However, studies of fur expression demonstrated that fur was not temperature regulated and that the hns651 mutation led to slightly decreased, rather than increased, fur mRNA levels at both temperatures (Table 2). While we cannot discount that the hns651 mutation might influence Fur protein levels or activity, this result raises the intriguing possibility that H-NS controls an unknown common intermediate that controls the fec, fhu, and fep systems. These findings may be particularly relevant to pathogenesis, given that these iron uptake systems are conserved in several strains of pathogenic E. coli and that H-NS is known to control genes within the uropathogenic E. coli yersiniabactin and salmochelin iron uptake systems (23).

H-NS regulates 72% of the genes more highly expressed at 23°C and regulates RpoS and DsrA levels to modulate RpoS-dependent gene expression. Our previous studies demonstrated that a low growth temperature of 23°C serves to increase and maintain 297 genes at a higher steady-state level of expression than does a growth temperature of 37°C (39), and the results presented here show that H-NS contributes to the regulation of 72% of these genes. Eighty-nine were RpoS-controlled genes associated with the general stress response, 19 genes were associated with biofilm development, and 17 were associated with the cold shock response (40), suggesting a strong linkage between these response mechanism pathways and H-NS (Table 1; see Table S2 in the supplemental material). More than 50% of the genes that are temperature regulated and H-NS controlled are of uncharacterized function, indicating there is much to be learned about adaptation to growth at low temperature.

Because more than 40% of the genes with increased expression at low temperature are RpoS and H-NS controlled, we investigated the effect of the hns651 mutation on transcription, both of the regulators themselves (RpoS and DsrA) and of a representative subset of genes whose expression at 23°C is known to be RpoS and DsrA dependent (40). RpoS levels are increased at low temperature by the small regulatory RNA DsrA (32), which alters rpoS mRNA secondary structure to allow more efficient rpoS translation (reviewed in references 16 and 30) and subsequent increased transcription of RpoS-dependent genes. In addition to the rpoS mRNA, DsrA has also been shown to target hns mRNA for degradation, subsequently decreasing H-NS levels (17). Thus, DsrA is thought to both increase RpoS and decrease H-NS protein levels to allow transcription of the general stress response genes at low temperature. In our studies of the wild-type strain, expression of rpoS and dsrA is increased at 23°C, consistent with previous studies indicating that their transcription is temperature regulated (Table 3) (29, 32). In the hns651 mutant strain, rpoS levels are not significantly altered, whereas levels of dsrA are significantly increased at both 37 and 23°C. To test whether the hns651 mutation leads to increased RpoS, protein levels were analyzed at 37 and 23°C in the wild-type and hns651 mutant strains by Western blotting (data not shown). While RpoS is present in both the wild-type and hns651 mutant strains at 23°C, it is only present at 37°C in the hns651 mutant and not in the wild-type strain. This result is consistent with the hypothesis that H-NS contributes to the thermoregulatory control of RpoS expression. It should be noted that separate studies demonstrated that in an hns mutant both the half-life of DsrA (2) and the stability of RpoS are increased (43) at 37°C.


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  		TABLE 3. RpoS- and DsrA-dependent gene mRNA levels at 37 and 23°C in wild-type and hns651 mutant strainsa

mRNA levels were measured at 37 and 23°C for a subset of genes whose expression at 23°C is RpoS and DsrA dependent in the wild-type and hns651 mutant strains (38). Genes associated with biofilm formation (bolA, csgA, and nhaR) and the cold shock response (otsA, yceP, and ycgZ) and genes with unknown function (ymdA and yhiM) were tested. At 37 and 23°C, otsA, yhiM, and yceP expression in the hns651 mutant equals or exceeds that observed at 23°C in the wild-type strain, indicating an exclusively repressive regulatory role for H-NS in the control of these operons at both 37 and 23°C. However, for bolA, ycgZ, ymdA, csgA, and nhaR, while the hns651 mutation leads to increased mRNA levels at 37°C in comparison to the wild-type strain at 37°C, they do not reach the maximal levels observed at 23°C. With the exception of bolA, this was true also at 23°C, where the hns651 mutant showed reduced expression levels of these genes in comparison to those in the wild-type strain at the same temperature. According to ChIP-chip analyses (27), the operons containing these genes are all direct targets of H-NS, suggesting that derepression at the nonpermissive temperature (37°C) in the hns651 mutant may be due, at least in part, to the loss of H-NS binding at these operons. Concomitant with this, hns651 also leads to increased RpoS levels at 37°C that could initiate the transcription of RpoS-dependent genes. However, for several of the genes tested, the effects on transcription cannot be attributed only to these two mechanisms as the absence of H-NS does not result in levels of expression at 37°C that match those seen at 23°C in the wild-type strain. The observation that these genes do not reach maximal levels in an hns651 mutant at either temperature argues that there are likely additional, indirect, effects of the hns651 mutant on other factors required for efficient transcription of these operons. Thus, no simple single regulatory role of H-NS can be invoked for the control of RpoS- and DsrA-dependent genes.

H-NS regulates gene expression at both temperatures. While H-NS controls a majority of thermoregulated genes, our study, along with others (reviewed in reference 24), indicates that H-NS frequently regulates gene expression at both temperatures rather than fitting an "all-or-nothing" model where it functions only at one temperature. Comparison of the hns651 mutant and wild-type expression ratios (Tables 2 and 3) of several iron utilization genes (fepC, fepD, fecI, fhuA, and fur) demonstrated similarly reduced transcription at both temperatures, while bolA, yceP, and yhiM were similarly derepressed by the hns651 mutation at 37 and 23°C, indicating an equivalent role for H-NS at both temperatures that may serve to modulate basal levels of transcription. In addition, the large number of nonthermoregulated genes controlled by H-NS (572) questions the characterization of H-NS as a molecular thermometer. However, H-NS-mediated repression is greater at 37°C than at 23°C for dsrA and otsA; the opposite is true for feoA, where repression by H-NS is greater at 23°C. Thus, at other operons, H-NS functions at both temperatures but with a more pronounced effect at one temperature. While they were less frequent, some genes (fecA, fhuE) showed expression patterns where the effect of H-NS was only observed at one temperature (37°C). Thus, our findings agree with other studies indicating that H-NS is present and influences transcription at both high and low temperatures, although in some cases (e.g., dsrA and feo) it appears that its repressive effect can be differentially modulated by temperature.

The authors of a recent study with Salmonella postulate that temperature and H-NS play an important role in niche-specific programming of virulence gene expression (5). In this model, temperature functions as an important top-level cue that prevents the production of virulence factors in nonhost environments, even when other environmental cues might lead to their expression. Our studies and others indicate that temperature and H-NS have a broader effect beyond virulence gene expression, acting to fine-tune and regulate a number of genes, allowing efficient colonization of the host and enabling appropriate adaptation to external environmental temperatures.


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ACKNOWLEDGMENTS
 
We thank the Genome Consortium for Active Teaching University and the University of Alberta for making microarrays available for use by undergraduate Talya Davis, who completed the microarray experiments in this study. We are grateful to present and former Smith College students, staff, and faculty for their technical assistance and advice, including Ying Mei, Abby Berns, Elyse Macksoud, Scott Edmands, Adam Hall, Nick Horton, and Rob Dorit.

This work was supported by the Albert F. Blakeslee Trust and by Smith College. Talya Davis was supported by an American Society for Microbiology undergraduate research fellowship.


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FOOTNOTES
 
* Corresponding author. Mailing address: Department of Biological Sciences, Smith College, Northampton, MA 01063. Phone: (413) 585-3815. Fax: (413) 585-3786. E-mail: cwhitezi{at}smith.edu Back

{triangledown} Published ahead of print on 14 November 2008. Back

{dagger} Supplemental material for this article may be found at http://jb.asm.org/. Back

{ddagger} Present address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111. Back


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Journal of Bacteriology, February 2009, p. 1106-1110, Vol. 191, No. 3
0021-9193/09/$08.00+0     doi:10.1128/JB.00599-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • White-Ziegler, C. A., Davis, T. R. (2009). Genome-Wide Identification of H-NS-Controlled, Temperature-Regulated Genes in Escherichia coli K-12. J. Bacteriol. 191: 1106-1110 [Abstract] [Full Text]  

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