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J Bacteriol. 1971 February; 105(2): 595-603
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Regulation of Chromosome Replication in Bacillus subtilis: Effects of Amino Acid Starvation in Strain W23

Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

ABSTRACT

Amino acid starvation allows limited synthesis of deoxyribonucleic acid (DNA) in Bacillus subtilis strain W23. DNA synthesis increased by about 30% after leucine starvation and by about 60% after histidine starvation. Genetic analysis on the DNA synthesized after amino acid starvation showed that all genetic markers examined have replicated, regardless of which amino acid was starved for. Initially, all markers replicated equally, but upon further replication, the thr cysB and the argA to lys regions replicated ahead of their neighboring, proximal regions. This could indicate that preferred stopping sites exist in these regions or additional sites from which replication can originate reside there. The results suggest that chromosome replication continues from those sites where it had stopped during amino acid starvation.

This article has been cited by other articles:

• Copeland, J. C. (1971). Regulation of Chromosome Replication in Bacillus subtilis: Marker Frequency Analysis after Amino Acid Starvation. Science 172: 159-161 [Abstract]