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J Bacteriol. 1971 July; 107(1): 95-99
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Chromosome Replication and the Division Cycle of Escherichia coli B/r

H. E. Kubitschek and M. L. Freedman1

a Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439

ABSTRACT

The average amount of deoxyribonucleic acid (DNA) per cell was measured in steady-state cultures of Escherichia coli B/r grown at 37 C in glucose-limited chemostats or in batch cultures in the exponential growth phase as maintained with one of several carbon sources. Within experimental errors, DNA content was dependent only on growth rate and independent of the type of culture, the carbon source, or the addition of growth factors. The amount of DNA per cell increased continuously with growth rate over the range of 0.02 to 3 divisions per hour. The data over the entire range of growth rates are in agreement with a constant time for a single replication point to traverse the entire genome, 47 min, and with cell division following 25 min after termination of replication. The measured amount of DNA per genome was 4.2 x 10–15 g (or 2.5 x 109 daltons).

FOOTNOTES

1 Present address: The University of Connecticut Health Center, Veterans Administration Hospital, Newington, Conn. 06111.

J Bacteriol. 1971 July; 107(1): 95-99
Copyright © 1971 American Society for Microbiology. All Rights Reserved.



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