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J Bacteriol. 1971 November; 108(2): 733-736
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Denitrification with Methanol: a Selective Enrichment for Hyphomicrobium Species

^a Department of Microbiology, The University of Texas at Austin, Austin, Texas 78712

ABSTRACT

Hyphomicrobium species were enriched in media with methanol as sole carbon source under conditions supporting denitrification. Pure cultures of Hyphomicrobium species were isolated which denitrified vigorously with methanol. Hyphomicrobium B522, isolated by aerobic enrichment, was adapted to anaerobic growth and denitrification. Hyphomicrobium B522 and a new isolate were surveyed for anaerobic growth and denitrification on a number of simple organic compounds. Cell suspensions were tested for denitrifying activity. Nitrogen production from nitrate and nitrite and carbon dioxide production from methanol were stoichiometric.

¹ Deceased 16 May 1971.

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