This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Low, B. Articles by Söll, D. Search for Related Content PubMed PubMed Citation Articles by Low, B. Articles by Söll, D.

 Previous Article  |  Next Article 

J Bacteriol. 1971 November; 108(2): 742-750
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases

Department of Molecular Biophysics and Biochemistry and Department of Radiology, Yale University, New Haven, Connecticut 06520

ABSTRACT

Two temperature-sensitive mutants of Escherichia coli have been found in which the conditional growth is a result of a thermosensitive leucyl-transfer ribonucleic acid (tRNA) synthetase and seryl-tRNA synthetase, respectively. The corresponding genetic loci, leuS and serS, cotransduce with lip and serC, respectively. As a result of the mutationally altered leucyl-tRNA synthetase, some leucine-, valine-, and isoleucine-forming enzymes were derepressed. Thus, leucyl-tRNA synthetase is involved in the repression of the enzymes needed for the synthesis of branched-chain amino acids.

This article has been cited by other articles:

• Karkhanis, V. A., Mascarenhas, A. P., Martinis, S. A. (2007). Amino Acid Toxicities of Escherichia coli That Are Prevented by Leucyl-tRNA Synthetase Amino Acid Editing. J. Bacteriol. 189: 8765-8768 [Abstract] [Full Text]  
• Karkhanis, V. A., Boniecki, M. T., Poruri, K., Martinis, S. A. (2006). A Viable Amino Acid Editing Activity in the Leucyl-tRNA Synthetase CP1-splicing Domain Is Not Required in the Yeast Mitochondria. J. Biol. Chem. 281: 33217-33225 [Abstract] [Full Text]  
• Milija, J., Lilic, M., Janjusevic, R., Jovanovic, G., Savic, D. J. (1999). tRNA Synthetase Mutants of Escherichia coli K-12 Are Resistant to the Gyrase Inhibitor Novobiocin. J. Bacteriol. 181: 2979-2983 [Abstract] [Full Text]