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J Bacteriol. 1971 December; 108(3): 1161-1167
Copyright © 1971 American Society for Microbiology. All Rights Reserved.

Fertility of Salmonella typhimurium Crosses with Escherichia coli

^a Department of Biology, McGill University, Montreal 110, Canada

ABSTRACT

At least one factor that causes low fertility of Salmonella typhimurium LT2 strains in crosses with Escherichia coli K-12 Hfr's can be inhibited by growing the female strains in supplemented minimal salts medium rather than in nutrient broth and by incubating the female strains at 50 C immediately before mating with the Hfr. These pretreatments can enhance the recovery of prototrophic recombinants for markers injected early by the Hfr by a factor of as much as 10⁴. The heat treatment is effective only on the female in intergeneric crosses and gradually loses (within 50 min) its effectiveness after return of heat-treated cells to 37 C. It is concluded that the restriction system of the female is heat-sensitive. Since markers injected late by the male enter females in which the heat-impaired restriction system has recovered, few recombinants for late markers are found. The presence of the leading end of an E. coli Hfr in an S. typhimurium-E. coli hybrid enhances by up to sevenfold the frequency of lac⁺ recombinants in subsequent crosses with an E. coli Hfr if the E. coli segment is integrated into the chromosome of the hybrid; the effect is less marked if the E. coli segment is not integrated.

¹ Present address: Polish Academy of Sciences, Institute of Biochemistry and Biophysics, Warszawa 12, Poland.

² Present address: Faculty of Medicine, Memorial University, St. John's, Newfoundland, Canada.