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J Bacteriol. 1972 April; 110(1): 306-312
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Chromosomal Localization of the Structural Genes of the Polypeptide Chain Elongation Factors

^a The Rockefeller University, New York, New York 10021, and Walter Reed Army Institute of Research, Washington, D.C. 20012

ABSTRACT

A survey of the polypeptide chain elongation factors in potentially sexually compatible genera was carried out. Factors from Escherichia coli and Proteus mirabilis were found to be clearly distinguishable by immunochemical and electrophoretic techniques. Mapping of the structural genes of these factors was undertaken by a study of the gene products in genetically defined E. coli-P. mirabilis hybrid diploid strains. It was found that the EF G factor mapped within 5 min of the streptomycin resistance locus, but the EF Ts factor did not map in this region.

¹ Present address: Friedrich Miescher Institute, Postfach 273, CH-4002 Basel, Switzerland.

² Present address: Max-Planck-Institut für Molekulare Genetik, 1 Berlin 33, Germany.