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J Bacteriol. 1972 October; 112(1): 220-223
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Activity of Deoxyribonucleic Acid Fragments of Defined Size in Bacillus subtilis Transformation

¹ Department of Biochemistry, Duke University, Durham, North Carolina 27710

ABSTRACT

The transforming activity of Bacillus subtilis deoxyribonucleic acid (DNA) that had been sheared and purified with respect to size by sucrose gradient sedimentation is given as a function of the DNA molecular weight. It is shown (i) that fragments of median molecular weight 1.2 x 10⁶ have finite activity (10^–4), (ii) that the shape of the activity-versus-molecular weight function is qualitatively similar to that observed previously for Diplococcus pneumoniae, and (iii) that this shape precludes interpretation in terms of critical size models.

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