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J Bacteriol. 1972 October; 112(1): 327-336
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Purification and Properties of the Adenosine Diphosphate-Glucose and Uridine Diphosphate-Glucose Pyrophosphorylases of Mycobacterium smegmatis: Inhibition and Activation of the Adenosine Diphosphate-Glucose Pyrophosphorylase

David Lapp1 and Alan D. Elbein

a Department of Biochemistry, The University of Texas Medical School, San Antonio, Texas 78229

ABSTRACT

Crude extracts of Mycobacterium smegmatis catalyzed the synthesis of adenosine diphosphate-glucose (ADP-Glc), cytidine diphosphate-glucose, guanosine diphosphate-glucose (GDP-Glc), thymidine diphosphate-glucose (TDP-Glc), and uridine diphosphate-glucose (UDP-Glc). In these crude enzyme fractions, high concentrations of trehalose-P inhibited the ADP-Glc and GDP-Glc pyrophosphorylases but did not effect the UDP-Glc or TDP-Glc pyrophosphorylases. Both the ADP-Glc pyrophosphorylase and the UDP-Glc pyrophosphorylase were partially purified (about 140-fold and 60-fold, respectively), and their properties were compared. For the ADP-Glc pyrophosphorylase, the Km for adenosine triphosphate was 6 x 10–4M, whereas that for glucose-1-P was 8 x 10–4M. The optimal concentration of Mg2+ was 1 x 10–3M, and the pH optimum was 8.5. For the UDP-Glc pyrophosphorylase, the Km for uridine triphosphate was 1 x 10–3M and for glucose-1-P was 2 x 10–3M. The optimal Mg2+ concentration was 1 x 10–3M, and the pH optimum was about 8.0. The purified ADP-Glc pyrophosphorylase was inhibited by fructose-6-P, fructose-1, 6-diphosphate, glucose-6-P, and phosphoenolpyruvate. On the other hand, trehalose, trehalose diphosphate, sodium pyruvate, and ribose-5-P did not effect the ADP-Glc pyrophosphorylase. None of these compounds, including trehalose-P, had any effect on the UDP-Glc pyrophosphorylase.

FOOTNOTES

1 Present address: Department of Cell and Molecular Biology, Medical College of Georgia, Augusta, Ga. 30902.

J Bacteriol. 1972 October; 112(1): 327-336
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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