This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Siegel, E. C. Search for Related Content PubMed PubMed Citation Articles by Siegel, E. C.

 Previous Article  |  Next Article 

J Bacteriol. 1973 January; 113(1): 161-166
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Ultraviolet-Sensitive Mutator mutU4 of Escherichia coli Inviable with polA

¹ Department of Biology, Tufts University, Medford, Massachusetts 02155

ABSTRACT

Attempts to transduce the ultraviolet-sensitive mutator lesion mutU4 into strains deficient in deoxyribonucleic acid polymerase I (polA) were unsuccessful. Mutator recombinants were found when the polA recipient had first been reverted to Pol⁺ by selection for resistance to methyl methanesulfonate. The inviability of the mutU4 polA double mutant was demonstrated by a reduction in the absolute number of transductants when the recipient was polA as compared with Pol⁺, and selection was made for markers very close to mutU4. Double mutants containing mutU4 and polA4, which determines a cold-sensitive polymerase, were unable to grow at 24 C, the nonpermissive temperature.

This article has been cited by other articles:

• Moolenaar, G. F., Moorman, C., Goosen, N. (2000). Role of the Escherichia coli Nucleotide Excision Repair Proteins in DNA Replication. J. Bacteriol. 182: 5706-5714 [Abstract] [Full Text]