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J Bacteriol. 1973 November; 116(2): 577-581
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Genetics and Biochemistry of Pyrimidine Biosynthesis in Bacillus subtilis: Linkage Between Mutations Resulting in a Requirement for Uracil

Raymond J. Kelleher Jr.1 and Harry Gooder

a Curriculum in Genetics and Department of Bacteriology and Immunology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514

ABSTRACT

A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipient of deoxyribonucleic acid extracted from phenotypically similar mutant strains. The results yielded data resulting in a fine-structure map in which all mutations were found to be linked in a small region corresponding to that previously published as the uracil region of the Bacillus subtilis genome.

FOOTNOTES

1 Present address: Salk Institute, San Diego, Calif. 92112.

J Bacteriol. 1973 November; 116(2): 577-581
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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