This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morris, D. R.
Right arrow Articles by Hansen, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morris, D. R.
Right arrow Articles by Hansen, M. T.

 Previous Article  |  Next Article 

J Bacteriol. 1973 November; 116(2): 588-592
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Influence of Polyamine Limitation on the Chain Growth Rates of ß-Galactosidase and of Its Messenger Ribonucleic Acid

David R. Morris1 and Mogens T. Hansen

a Universitetes Mikrobiologiske Institut, Copenhagen, Denmark

ABSTRACT

The rates of elongation of ß-galactosidase and its messenger ribonucleic acid (RNA) were estimated in a polyamine-deficient mutant of Escherichia coli through an analysis of the kinetics of enzyme induction. The chain growth of ß-galactosidase was calculated from the time required after the appearance of an amino terminal fragment of 60 amino acids (auto-{alpha}) until completed enzyme began to accumulate. The elongation rate of ß-galactosidase messenger RNA was estimated from the time after induction at which streptolydigen-resistant, enzyme-forming capacity first appeared. Upon polyamine starvation, the rate of polypeptide elongation slowed from 17 to 10 amino acids per s and the messenger RNA elongation rate decreased from 47 to 30 nucleotides per s. These reductions in polymerization rates were proportional to the decrease in cellular growth rate produced by polyamine starvation. It was concluded that, although it is quite unlikely that polyamine levels are involved in regulation of cell growth, they may be acting as cofactors in the synthesis of RNA or protein, or both.

FOOTNOTES

1 Present address: Department of Biochemistry SJ-70, University of Washington, Seattle, Wash. 98195.

J Bacteriol. 1973 November; 116(2): 588-592
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»