This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alper, M D
Right arrow Articles by Ames, B N
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alper, M D
Right arrow Articles by Ames, B N

 Previous Article  |  Next Article 

J Bacteriol. 1978 January; 133(1): 149-157

Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: positive selection of Salmonella typhimurium cya and crp mutants.

M D Alper and B N Ames

ABSTRACT

Mutants in the cyclic AMP (cAMP) control system in Salmonella typhimurium (cya = adenyl cyclase, crp = cAMP receptor protein) were partially resistant to growth inhibition by 22 antibiotics (including fosfomycin, nalidixic acid, and streptomycin) and 29 inhibitory analogs of normal bacterial fuel/carbon sources. This resistance was used as the basis for an efficient positive selection of cya and crp mutants. We propose that these antibiotics and analogs enter the bacteria through transport systems normally used for transporting fuel/carbon sources and that this is accomplished because of a structural similarity between the antibiotic and the natural substrate of the particular transport system involved. We propose that these transport systems are all under positive control by cAMP and that cAMP acts as a signal molecule (alarmone) for fuel/carbon deprivation. Evidence is provided for a hierarchy within operons controlled by cAMP. The methodology is shown to be useful for analyzing both antibiotic transport systems and the cAMP super-control system.

J Bacteriol. 1978 January; 133(1): 149-157




This article has been cited by other articles:

  • Castaneda-Garcia, A., Rodriguez-Rojas, A., Guelfo, J. R., Blazquez, J. (2009). The Glycerol-3-Phosphate Permease GlpT Is the Only Fosfomycin Transporter in Pseudomonas aeruginosa. J. Bacteriol. 191: 6968-6974 [Abstract] [Full Text]  
  • Walker, K. A., Mallik, P., Pratt, T. S., Osuna, R. (2004). The Escherichia coli fis Promoter Is Regulated by Changes in the Levels of Its Transcription Initiation Nucleotide CTP. J. Biol. Chem. 279: 50818-50828 [Abstract] [Full Text]  
  • Buurman, E. T., McLaggan, D., Naprstek, J., Epstein, W. (2004). Multiple Paths for Nonphysiological Transport of K+ in Escherichia coli. J. Bacteriol. 186: 4238-4245 [Abstract] [Full Text]  
  • Guvener, Z. T., McCarter, L. L. (2003). Multiple Regulators Control Capsular Polysaccharide Production in Vibrio parahaemolyticus. J. Bacteriol. 185: 5431-5441 [Abstract] [Full Text]  
  • Nilsson, A. I., Berg, O. G., Aspevall, O., Kahlmeter, G., Andersson, D. I. (2003). Biological Costs and Mechanisms of Fosfomycin Resistance in Escherichia coli. Antimicrob. Agents Chemother. 47: 2850-2858 [Abstract] [Full Text]  
  • Stewart, B. J., McCarter, L. L. (2003). Lateral Flagellar Gene System of Vibrio parahaemolyticus. J. Bacteriol. 185: 4508-4518 [Abstract] [Full Text]  
  • Walker, K. A., Osuna, R. (2002). Factors Affecting Start Site Selection at the Escherichia coli fis Promoter. J. Bacteriol. 184: 4783-4791 [Abstract] [Full Text]  
  • Tellez-Sosa, J., Soberon, N., Vega-Segura, A., Torres-Marquez, M. E., Cevallos, M. A. (2002). The Rhizobium etli cyaC Product: Characterization of a Novel Adenylate Cyclase Class. J. Bacteriol. 184: 3560-3568 [Abstract] [Full Text]  
  • Bochner, B. R., Gadzinski, P., Panomitros, E. (2001). Phenotype MicroArrays for High-Throughput Phenotypic Testing and Assay of Gene Function. Genome Res 11: 1246-1255 [Abstract] [Full Text]  
  • Cheng, Y., Dylla, S. M., Turnbough, C. L. Jr. (2001). A Long T {middle dot} A Tract in the upp Initially Transcribed Region Is Required for Regulation of upp Expression by UTP-Dependent Reiterative Transcription in Escherichia coli. J. Bacteriol. 183: 221-228 [Abstract] [Full Text]  
  • Pokholok, D. K., Redlak, M., Turnbough, C. L. Jr., Dylla, S., Holmes, W. M. (1999). Multiple Mechanisms Are Used for Growth Rate and Stringent Control of leuV Transcriptional Initiation in Escherichia coli. J. Bacteriol. 181: 5771-5782 [Abstract] [Full Text]  
  • Kennedy, M. J., Yancey, R. J. Jr., Sanchez, M. S., Rzepkowski, R. A., Kelly, S. M., Curtiss, R. III (1999). Attenuation and Immunogenicity of Delta cya Delta crp Derivatives of Salmonella choleraesuis in Pigs. Infect. Immun. 67: 4628-4636 [Abstract] [Full Text]  
  • Karimova, G., Pidoux, J., Ullmann, A., Ladant, D. (1998). A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc. Natl. Acad. Sci. USA 95: 5752-5756 [Abstract] [Full Text]  
  • Han, X., Turnbough, C. L. Jr. (1998). Regulation of carAB Expression in Escherichia coli Occurs in Part through UTP-Sensitive Reiterative Transcription. J. Bacteriol. 180: 705-713 [Abstract] [Full Text]  
  • Gaal, T., Bartlett, M. S., Ross, W., Turnbough Jr., C. L., Gourse, R. L. (1997). Transcription Regulation by Initiating NTP Concentration: rRNA Synthesis in Bacteria. Science 278: 2092-2097 [Abstract] [Full Text]  
  • Liu, C, Heath, L S, Turnbough, C L (1994). Regulation of pyrBI operon expression in Escherichia coli by UTP-sensitive reiterative RNA synthesis during transcriptional initiation.. Genes Dev. 8: 2904-2912 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»