This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Santos, T Articles by Nombela, C Search for Related Content PubMed PubMed Citation Articles by Santos, T Articles by Nombela, C

 Previous Article  |  Next Article 

J Bacteriol. 1979 August; 139(2): 333-338

Saccharomyces cerevisiae mutant defective in exo-1,3-beta-glucanase production.

T Santos, F del Rey, J Conde, J R Villanueva and C Nombela

ABSTRACT

Saccharomyces cerevisiae S288C produced two laminarinases (1,3-beta-glucanases) which were separated by diethylaminoethyl-Sephadex column chromatography; one was an endo-1,3-beta-glucanase, and the other was an exo-1,3-beta-glucanase active not only on laminarin but also on pustulan (1,6-beta-glucan) and on p-nitrophenyl-beta-D-glucoside. A mutant defective in the production of this last enzyme was isolated, and the mutation was named exb1-1. The selection procedure was based on the capacity of exo-1,3-beta-glucanase to hydrolyze synthetic glucosides. The level of endo-1,3-beta-glucanase in cell extracts of the mutant was normal, but the exo-1,3-beta-glucanase could not be detected by column chromatographic analysis of these extracts. The mutant phenotype, recessive in heterozygous diploids, was stable through successive meioses and showed a Mendelian segregation, indicating that the mutation affected a single gene, which was named EXB1. The lack of production of exo-1,3-beta-glucanase persisted through all the phases of growth, but growth itself was not impaired by the enzyme deficiency.

---

J Bacteriol. 1979 August; 139(2): 333-338

This article has been cited by other articles:

• Lustgarten, V., Gerst, J. E. (1999). Yeast VSM1 Encodes a v-SNARE Binding Protein That May Act as a Negative Regulator of Constitutive Exocytosis. Mol. Cell. Biol. 19: 4480-4494 [Abstract] [Full Text]  
• David, D., Sundarababu, S., Gerst, J. E. (1998). Involvement of Long Chain Fatty Acid Elongation in the Trafficking of Secretory Vesicles in Yeast. JCB 143: 1167-1182 [Abstract] [Full Text]