This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Herrero, A Articles by Guerrero, M G Search for Related Content PubMed PubMed Citation Articles by Herrero, A Articles by Guerrero, M G

 Previous Article  |  Next Article 

J Bacteriol. 1981 January; 145(1): 175-180

Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719.

ABSTRACT

The effect of the nitrogen source on the cellular activity of ferredoxin-nitrate reductase in different cyanobacteria was examined. In the unicellular species Anacystis nidulans, nitrate reductase was repressed in the presence of ammonium but de novo enzyme synthesis took place in media containing either nitrate or not nitrogen source, indicating that nitrate was not required as an obligate inducer. Nitrate reductase in A. nidulans was freed from ammonium repression by L-methionine-D,L-sulfoximine, an irreversible inhibitor of glutamine synthetase. Ammonium-promoted repression appears therefore to be indirect; ammonium has to be metabolized through glutamine synthetase to be effective in the repression of nitrate reductase. Unlike the situation in A. nidulans, nitrate appeared to play an active role in nitrate reductase synthesis in the filamentous nitrogen-fixing strains Anabaena sp. strain 7119 and Nostoc sp. strain 6719, with ammonium acting as an antagonist with regard to nitrate.

This article has been cited by other articles:

• Rajaram, H., Apte, S. K. (2008). Nitrogen status and heat-stress-dependent differential expression of the cpn60 chaperonin gene influences thermotolerance in the cyanobacterium Anabaena. Microbiology 154: 317-325 [Abstract] [Full Text]  
• Zhang, Y., Pu, H., Wang, Q., Cheng, S., Zhao, W., Zhang, Y., Zhao, J. (2007). PII Is Important in Regulation of Nitrogen Metabolism but Not Required for Heterocyst Formation in the Cyanobacterium Anabaena sp. PCC 7120. J. Biol. Chem. 282: 33641-33648 [Abstract] [Full Text]  
• Takatani, N., Omata, T. (2006). Effects of PII Deficiency on Expression of the Genes Involved in Ammonium Utilization in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Plant Cell Physiol 47: 679-688 [Abstract] [Full Text]  
• Kolodny, N. H., Bauer, D., Bryce, K., Klucevsek, K., Lane, A., Medeiros, L., Mercer, W., Moin, S., Park, D., Petersen, J., Wright, J., Yuen, C., Wolfson, A. J., Allen, M. M. (2006). Effect of Nitrogen Source on Cyanophycin Synthesis in Synechocystis sp. Strain PCC 6308. J. Bacteriol. 188: 934-940 [Abstract] [Full Text]  
• Kloft, N., Forchhammer, K. (2005). Signal Transduction Protein PII Phosphatase PphA Is Required for Light-Dependent Control of Nitrate Utilization in Synechocystis sp. Strain PCC 6803. J. Bacteriol. 187: 6683-6690 [Abstract] [Full Text]  
• Kobayashi, M., Takatani, N., Tanigawa, M., Omata, T. (2005). Posttranslational Regulation of Nitrate Assimilation in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. Bacteriol. 187: 498-506 [Abstract] [Full Text]  
• Aichi, M., Maeda, S.-I., Ichikawa, K., Omata, T. (2004). Nitrite-Responsive Activation of the Nitrate Assimilation Operon in Cyanobacteria Plays an Essential Role in Up-Regulation of Nitrate Assimilation Activities under Nitrate-Limited Growth Conditions. J. Bacteriol. 186: 3224-3229 [Abstract] [Full Text]  
• Maeda, S.-i., Omata, T. (2004). A Novel Gene (narM) Required for Expression of Nitrate Reductase Activity in the Cyanobacterium Synechococcus elongatus Strain PCC7942. J. Bacteriol. 186: 2107-2114 [Abstract] [Full Text]  
• Wang, T.-H., Fu, H., Shieh, Y.-J. (2003). Monomeric NarB Is a Dual-Affinity Nitrate Reductase, and Its Activity Is Regulated Differently from That of Nitrate Uptake in the Unicellular Diazotrophic Cyanobacterium Synechococcus sp. Strain RF-1. J. Bacteriol. 185: 5838-5846 [Abstract] [Full Text]  
• Aichi, M., Takatani, N., Omata, T. (2001). Role of NtcB in Activation of Nitrate Assimilation Genes in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. Bacteriol. 183: 5840-5847 [Abstract] [Full Text]  
• Lindell, D., Post, A. F. (2001). Ecological Aspects of ntcA Gene Expression and Its Use as an Indicator of the Nitrogen Status of Marine Synechococcus spp.. Appl. Environ. Microbiol. 67: 3340-3349 [Abstract] [Full Text]  
• Miller, S. R., Castenholz, R. W. (2001). Ecological Physiology of Synechococcus sp. Strain SH-94-5, a Naturally Occurring Cyanobacterium Deficient in Nitrate Assimilation. Appl. Environ. Microbiol. 67: 3002-3009 [Abstract] [Full Text]  
• Herrero, A., Muro-Pastor, A. M., Flores, E. (2001). Nitrogen Control in Cyanobacteria. J. Bacteriol. 183: 411-425 [Full Text]  
• Chávez, S., Lucena, J. M., Reyes, J. C., Florencio, F. J., Candau, P. (1999). The Presence of Glutamate Dehydrogenase Is a Selective Advantage for the Cyanobacterium Synechocystis sp. Strain PCC 6803 under Nonexponential Growth Conditions. J. Bacteriol. 181: 808-813 [Abstract] [Full Text]  
• Sakamoto, T., Bryant, D. A. (1999). Nitrate Transport and Not Photoinhibition Limits Growth of the Freshwater Cyanobacterium Synechococcus Species PCC 6301 at Low Temperature. Plant Physiol. 119: 785-794 [Abstract] [Full Text]  
• Maeda, S.-i., Okamura, M., Kobayashi, M., Omata, T. (1998). Nitrite-Specific Active Transport System of the Cyanobacterium Synechococcus sp. Strain PCC 7942. J. Bacteriol. 180: 6761-6763 [Abstract] [Full Text]  
• Lindell, D., Padan, E., Post, A. F. (1998). Regulation of ntcA Expression and Nitrite Uptake in the Marine Synechococcus sp. Strain WH 7803. J. Bacteriol. 180: 1878-1886 [Abstract] [Full Text]  
• Kobayashi, M., Rodriguez, R., Lara, C., Omata, T. (1997). Involvement of the C-terminal Domain of an ATP-binding Subunit in the Regulation of the ABC-type Nitrate/Nitrite Transporter of the Cyanobacterium Synechococcus sp. Strain PCC 7942. J. Biol. Chem. 272: 27197-27201 [Abstract] [Full Text]  
• Maeda, S.-i., Omata, T. (1997). Substrate-binding Lipoprotein of the Cyanobacterium Synechococcus sp. Strain PCC 7942Involved in the Transport of Nitrate and Nitrite. J. Biol. Chem. 272: 3036-3041 [Abstract] [Full Text]  
• Muro-Pastor, M. I., Reyes, J. C., Florencio, F. J. (2001). Cyanobacteria Perceive Nitrogen Status by Sensing Intracellular 2-Oxoglutarate Levels. J. Biol. Chem. 276: 38320-38328 [Abstract] [Full Text]