This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Imanaka, T Articles by Aiba, S Search for Related Content PubMed PubMed Citation Articles by Imanaka, T Articles by Aiba, S

 Previous Article  |  Next Article 

J Bacteriol. 1981 June; 146(3): 1091-1097

Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids.

ABSTRACT

Ten plasmids were isolated as covalently closed circular deoxyribonucleic acid from antibiotic-resistant thermophilic bacteria. Of the 10 plasmids tested, 2 could transform Bacillus subtilis, yielding resistance to specific antibiotics. Plasmid pTB20 (2.8 X 10(6) daltons, approximately 24 copies per chromosome) specifies resistance to tetracycline (Tcr), whereas pTB19 (17.2 X 10(6) daltons, approximately 1 copy per chromosome) renders the host resistant to both kanamycin and tetracycline (KMrTcr). Three plasmids were not self-transmissible. The restriction endonuclease cleavage maps of the two plasmids, pTB19 and pTB20, were constructed. pTB19 and pTB20, both of which were originally isolated from thermophilic bacilli, were tested for stability in B. subtilis. Digestion of pTB19 followed by ligation yielded deletion plasmids pTB512 (Kmr), pTB52 (Tcr), and pTB53 (KmrTcr). Determinants of Kmr, Tcr, and DNA replication were associated with EcoRI fragments R1b (4.2 X 10(6) daltons), R3 (2.8 X 10(6) daltons), and R1a (4.2 X 10(6) daltons), respectively. Restriction endonuclease cleavage maps of pTB51, pTB52, and pTB53 were constructed. Tetracycline resistance of pTB20 was confirmed to be in the EcoRI fragment (1.85 X 10(6) daltons).

This article has been cited by other articles:

• Ohtani, N., Haruki, M., Morikawa, M., Kanaya, S. (2001). Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis. Protein Eng Des Sel 14: 975-982 [Abstract] [Full Text]  
• Tsuge, K., Itaya, M. (2001). Recombinational Transfer of 100-Kilobase Genomic DNA to Plasmid in Bacillus subtilis 168. J. Bacteriol. 183: 5453-5458 [Abstract] [Full Text]  
• Ueda, K., Ohno, M., Yamamoto, K., Nara, H., Mori, Y., Shimada, M., Hayashi, M., Oida, H., Terashima, Y., Nagata, M., Beppu, T. (2001). Distribution and Diversity of Symbiotic Thermophiles, Symbiobacterium thermophilum and Related Bacteria, in Natural Environments. Appl. Environ. Microbiol. 67: 3779-3784 [Abstract] [Full Text]