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J Bacteriol. 1984 December; 160(3): 1181-1183

Simple, rapid, and quantitative release of periplasmic proteins by chloroform.

G F Ames, C Prody and S Kustu

ABSTRACT

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.

J Bacteriol. 1984 December; 160(3): 1181-1183




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