This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tsuruoka, T Articles by Matsuhashi, M Search for Related Content PubMed PubMed Citation Articles by Tsuruoka, T Articles by Matsuhashi, M

 Previous Article  |  Next Article 

J Bacteriol. 1988 November; 170(11): 5229-5235

research-article

Thermosensitive omsA mutation of Escherichia coli that causes thermoregulated release of periplasmic proteins.

T Tsuruoka, M Ito, S Tomioka, A Hirata and M Matsuhashi

Central Research Laboratories, Meiji Seika, Ltd., Yokohama, Japan.

ABSTRACT

A mutant of Escherichia coli with a thermosensitive defect, possibly in the outer membrane (omsA mutant), was isolated from E. coli K-12 by mutagenization and selection for thermosensitivity and beta-lactam supersensitivity of growth. The mutant also showed very high sensitivity to other antibiotics, such as macarbomycin, midecamycin, rifampin, and bacitracin. The mutation was recessive to the wild type and was mapped at about 4 min on the E. coli chromosome between fhuA and metD. The mutation caused rapid release into the medium of periplasmic enzymes such as RTEM penicillinase but practically no cytoplasmic enzyme when cells grown at 30 degrees C were transferred to 37 or 42 degrees C. Electron microscopic observations showed many large double-layered vesicles attached to the surface of cells incubated at 42 degrees C. We conclude that the mutant had a mutation that caused a temperature-dependent defect in the outer membrane structure or its assembly (named an oms mutation). The omsA mutant may be useful for production of periplasmic proteins, which it releases into the culture medium on shift up of temperature.

---

J Bacteriol. 1988 November; 170(11): 5229-5235

This article has been cited by other articles:

• De Lay, N. R., Cronan, J. E. (2008). Genetic Interaction Between the Escherichia coli AcpT Phosphopantetheinyl Transferase and the YejM Inner Membrane Protein. Genetics 178: 1327-1337 [Abstract] [Full Text]  
• Kuroda, T., Okuda, N., Saitoh, N., Hiyama, T., Terasaki, Y., Anazawa, H., Hirata, A., Mogi, T., Kusaka, I., Tsuchiya, T., Yabe, I. (1998). Patch Clamp Studies on Ion Pumps of the Cytoplasmic Membrane of Escherichia coli. FORMATION, PREPARATION, AND UTILIZATION OF GIANT VACUOLE-LIKE STRUCTURES CONSISTING OF EVERTED CYTOPLASMIC MEMBRANE. J. Biol. Chem. 273: 16897-16904 [Abstract] [Full Text]