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J Bacteriol. 1988 December; 170(12): 5433-5439

research-article

Biochemical and genetic analysis of hydrogen metabolism in Escherichia coli: the hydB gene.

P Sankar and K T Shanmugam

Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.

ABSTRACT

Production of active hydrogenase by Escherichia coli requires several gene products. One of the essential genes, hydB, is encoded by a DNA fragment of approximately 1.0 kilobase. The hydB gene produced a protein with an apparent molecular weight of 32,000. The hydB gene was transcribed only under anaerobic conditions. Oxygen and nitrate repressed transcription of this gene. hydB gene transcription also required sigma 60, the product of the rpoN gene.

J Bacteriol. 1988 December; 170(12): 5433-5439






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