This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ebbole, D J
Right arrow Articles by Zalkin, H
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ebbole, D J
Right arrow Articles by Zalkin, H

 Previous Article  |  Next Article 

J Bacteriol. 1989 April; 171(4): 2136-2141

research-article

Bacillus subtilis pur operon expression and regulation.

D J Ebbole and H Zalkin

Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.

ABSTRACT

The Bacillus subtilis pur operon is a 12-gene cluster, purEKB-purC(orf)QLF-purMNH(J)-purD, organized in groups of overlapping coding units separated by intercistronic gaps. Translational fusions of Escherichia coli lacZ were constructed to purE, purC, and purM, the first gene of each group. Analyses of gene fusions integrated into the chromosomal pur operon exclude the possibility of internal promoters in intercistronic regions and support the view that transcription is from the single sigma 43 promoter at the 5' end of the operon. Enzyme and mRNA measurements indicate that transcriptional regulation occurs solely at the 5' end of the operon. The relative levels of beta-galactosidase from purE-lacZ, purC-lacZ, and purM-lacZ were determined under repressing and nonrepressing conditions. These results indicate that expression of purC-lacZ was 3.0- to 6.8-fold higher than purE-lacZ because of enhanced translational efficiency. The enhanced translational efficiency of purC-lacZ was accompanied by a partial escape from regulation by purines. This anomalous effect on purC-lacZ was the only suggestion for posttranscriptional regulation.

J Bacteriol. 1989 April; 171(4): 2136-2141




This article has been cited by other articles:

  • Fox, P. M., Climo, M. W., Archer, G. L. (2007). Lack of Relationship between Purine Biosynthesis and Vancomycin Resistance in Staphylococcus aureus: a Cautionary Tale for Microarray Interpretation. Antimicrob. Agents Chemother. 51: 1274-1280 [Abstract] [Full Text]  
  • Arsene-Ploetze, F., Nicoloff, H., Kammerer, B., Martinussen, J., Bringel, F. (2006). Uracil Salvage Pathway in Lactobacillus plantarum: Transcription and Genetic Studies. J. Bacteriol. 188: 4777-4786 [Abstract] [Full Text]  
  • Sinha, S. C., Krahn, J., Shin, B. S., Tomchick, D. R., Zalkin, H., Smith, J. L. (2003). The Purine Repressor of Bacillus subtilis: a Novel Combination of Domains Adapted for Transcription Regulation. J. Bacteriol. 185: 4087-4098 [Abstract] [Full Text]  
  • Bera, A. K., Zhu, J., Zalkin, H., Smith, J. L. (2003). Functional Dissection of the Bacillus subtilis pur Operator Site. J. Bacteriol. 185: 4099-4109 [Abstract] [Full Text]  
  • Buendia-Claveria, A. M., Moussaid, A., Ollero, F. J., Vinardell, J. M., Torres, A., Moreno, J., Gil-Serrano, A. M., Rodriguez-Carvajal, M. A., Tejero-Mateo, P., Peart, J. L., Brewin, N. J., Ruiz-Sainz, J. E. (2003). A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology 149: 1807-1818 [Abstract] [Full Text]  
  • Saxild, H. H., Nygaard, P. (2000). The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis. Microbiology 146: 807-814 [Abstract] [Full Text]  
  • Rappu, P., Shin, B. S., Zalkin, H., Mäntsälä, P. (1999). A Role for a Highly Conserved Protein of Unknown Function in Regulation of Bacillus subtilis purA by the Purine Repressor. J. Bacteriol. 181: 3810-3815 [Abstract] [Full Text]  
  • Overbeek, R., Fonstein, M., D'Souza, M., Pusch, G. D., Maltsev, N. (1999). The use of gene clusters to infer functional coupling. Proc. Natl. Acad. Sci. USA 96: 2896-2901 [Abstract] [Full Text]  
  • Kilstrup, M., Jessing, S. G., Wichmand-Jørgensen, S. B., Madsen, M., Nilsson, D. (1998). Activation Control of pur Gene Expression in Lactococcus lactis: Proposal for a Consensus Activator Binding Sequence Based on Deletion Analysis and Site-Directed Mutagenesis of purC and purD Promoter Regions. J. Bacteriol. 180: 3900-3906 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»