This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grundy, F J Articles by Henkin, T M Search for Related Content PubMed PubMed Citation Articles by Grundy, F J Articles by Henkin, T M

 Previous Article  |  Next Article 

J Bacteriol. 1991 August; 173(15): 4595-4602

research-article

The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis.

F J Grundy and T M Henkin

Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130.

ABSTRACT

Although the mechanisms for regulation of ribosomal protein gene expression have been established for gram-negative bacteria such as Escherichia coli, the regulation of these genes in gram-positive bacteria such as Bacillus subtilis has not yet been characterized. In this study, the B. subtilis rpsD gene, encoding ribosomal protein S4, was found to be subject to autogenous control. In E. coli, rpsD is located in the alpha operon, and S4 acts as the translational regulator for alpha operon expression, binding to a target site in the alpha operon mRNA. The target site for repression of B. subtilis rpsD by protein S4 was localized by deletion and oligonucleotide-directed mutagenesis to the leader region of the monocistronic rpsD gene. The B. subtilis rpsD leader exhibits little sequence homology to the E. coli alpha operon leader but may be able to form a pseudoknotlike structure similar to that found in E. coli.

---

J Bacteriol. 1991 August; 173(15): 4595-4602

This article has been cited by other articles:

• Choonee, N., Even, S., Zig, L., Putzer, H. (2007). Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism. Nucleic Acids Res 35: 1578-1588 [Abstract] [Full Text]  
• Ng, W.-L., Kazmierczak, K. M., Robertson, G. T., Gilmour, R., Winkler, M. E. (2003). Transcriptional Regulation and Signature Patterns Revealed by Microarray Analyses of Streptococcus pneumoniae R6 Challenged with Sublethal Concentrations of Translation Inhibitors. J. Bacteriol. 185: 359-370 [Abstract] [Full Text]  
• Allen, T., Shen, P., Samsel, L., Liu, R., Lindahl, L., Zengel, J. M. (1999). Phylogenetic Analysis of L4-Mediated Autogenous Control of the S10 Ribosomal Protein Operon. J. Bacteriol. 181: 6124-6132 [Abstract] [Full Text]