This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Massidda, O
Right arrow Articles by Satta, G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Massidda, O
Right arrow Articles by Satta, G
Right arrowPubmed/NCBI databases
*Protein
*Substance via MeSH

 Previous Article  |  Next Article 

J Bacteriol. 1991 August; 173(15): 4611-4617

research-article

The Aeromonas hydrophila cphA gene: molecular heterogeneity among class B metallo-beta-lactamases.

O Massidda, G M Rossolini and G Satta

Dipartimento di Biologia Molecolare, Sezione Microbiologia, Universitá degli Studi di Siena, Italy.

ABSTRACT

An Aeromonas hydrophila gene, named cphA, coding for a carbapenem-hydrolyzing metallo-beta-lactamase, was cloned in Escherichia coli by screening an Aeromonas genomic library for clones able to grow on imipenem-containing medium. From sequencing data, the cloned cphA gene appeared able to code for a polypeptide of 254 amino acids whose sequence includes a potential N-terminal leader sequence for targeting the protein to the periplasmic space. These data were in agreement with the molecular mass of the original Aeromonas enzyme and of the recombinant enzyme produced in E. coli, evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of crude beta-lactamase preparations followed by renaturation treatment for proteins separated in the gel and localization of protein bands showing carbapenem-hydrolyzing beta-lactamase activity by a modified iodometric technique. The deduced amino acid sequence of the CphA enzyme showed regions of partial homology with both the beta-lactamase II of Bacillus cereus and the CfiA beta-lactamase of Bacteroides fragilis. Sequence homologies were more pronounced in the regions encompassing the amino acid residues known in the enzyme of B. cereus to function as ligand-binding residues for the metal cofactor. The CphA enzyme, however, appeared to share a lower degree of similarity with the two other enzymes, which, in turn, seemed more closely related to each other. These results, therefore, suggest the existence of at least two molecular subclasses within molecular class B metallo-beta-lactamases.

J Bacteriol. 1991 August; 173(15): 4611-4617




This article has been cited by other articles:

  • Stoczko, M., Frere, J.-M., Rossolini, G. M., Docquier, J.-D. (2008). Functional Diversity among Metallo-{beta}-Lactamases: Characterization of the CAR-1 Enzyme of Erwinia carotovora. Antimicrob. Agents Chemother. 52: 2473-2479 [Abstract] [Full Text]  
  • Seshadri, R., Joseph, S. W., Chopra, A. K., Sha, J., Shaw, J., Graf, J., Haft, D., Wu, M., Ren, Q., Rosovitz, M. J., Madupu, R., Tallon, L., Kim, M., Jin, S., Vuong, H., Stine, O. C., Ali, A., Horneman, A. J., Heidelberg, J. F. (2006). Genome Sequence of Aeromonas hydrophila ATCC 7966T: Jack of All Trades. J. Bacteriol. 188: 8272-8282 [Abstract] [Full Text]  
  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  
  • Furushita, M., Okamoto, A., Maeda, T., Ohta, M., Shiba, T. (2005). Isolation of Multidrug-Resistant Stenotrophomonas maltophilia from Cultured Yellowtail (Seriola quinqueradiata) from a Marine Fish Farm. Appl. Environ. Microbiol. 71: 5598-5600 [Abstract] [Full Text]  
  • Bebrone, C., Anne, C., De Vriendt, K., Devreese, B., Rossolini, G. M., Van Beeumen, J., Frere, J.-M., Galleni, M. (2005). Dramatic Broadening of the Substrate Profile of the Aeromonas hydrophila CphA Metallo-{beta}-lactamase by Site-directed Mutagenesis. J. Biol. Chem. 280: 28195-28202 [Abstract] [Full Text]  
  • Yamaguchi, Y., Kuroki, T., Yasuzawa, H., Higashi, T., Jin, W., Kawanami, A., Yamagata, Y., Arakawa, Y., Goto, M., Kurosaki, H. (2005). Probing the Role of Asp-120(81) of Metallo-{beta}-lactamase (IMP-1) by Site-directed Mutagenesis, Kinetic Studies, and X-ray Crystallography. J. Biol. Chem. 280: 20824-20832 [Abstract] [Full Text]  
  • Walsh, T. R., Toleman, M. A., Poirel, L., Nordmann, P. (2005). Metallo-{beta}-Lactamases: the Quiet before the Storm?. Clin. Microbiol. Rev. 18: 306-325 [Abstract] [Full Text]  
  • Garau, G., Garcia-Saez, I., Bebrone, C., Anne, C., Mercuri, P., Galleni, M., Frere, J.-M., Dideberg, O. (2004). Update of the Standard Numbering Scheme for Class B {beta}-Lactamases. Antimicrob. Agents Chemother. 48: 2347-2349 [Full Text]  
  • Saavedra, M. J., Peixe, L., Sousa, J. C., Henriques, I., Alves, A., Correia, A. (2003). Sfh-I, a Subclass B2 Metallo-{beta}-Lactamase from a Serratia fonticola Environmental Isolate. Antimicrob. Agents Chemother. 47: 2330-2333 [Abstract] [Full Text]  
  • Niumsup, P., Simm, A. M., Nurmahomed, K., Walsh, T. R., Bennett, P. M., Avison, M. B. (2003). Genetic linkage of the penicillinase gene, amp, and blrAB, encoding the regulator of {beta}-lactamase expression in Aeromonas spp.. J Antimicrob Chemother 51: 1351-1358 [Abstract] [Full Text]  
  • Heinz, U., Bauer, R., Wommer, S., Meyer-Klaucke, W., Papamichaels, C., Bateson, J., Adolph, H.-W. (2003). Coordination Geometries of Metal Ions in D- or L-Captopril-inhibited Metallo-{beta}-lactamases. J. Biol. Chem. 278: 20659-20666 [Abstract] [Full Text]  
  • Murphy, T. A., Simm, A. M., Toleman, M. A., Jones, R. N., Walsh, T. R. (2003). Biochemical Characterization of the Acquired Metallo-{beta}-Lactamase SPM-1 from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 47: 582-587 [Abstract] [Full Text]  
  • Naas, T., Bellais, S., Nordmann, P. (2003). Molecular and biochemical characterization of a carbapenem-hydrolysing {beta}-lactamase from Flavobacterium johnsoniae. J Antimicrob Chemother 51: 267-273 [Abstract] [Full Text]  
  • Mammeri, H., Bellais, S., Nordmann, P. (2002). Chromosome-Encoded {beta}-Lactamases TUS-1 and MUS-1 from Myroides odoratus and Myroides odoratimimus (Formerly Flavobacterium odoratum), New Members of the Lineage of Molecular Subclass B1 Metalloenzymes. Antimicrob. Agents Chemother. 46: 3561-3567 [Abstract] [Full Text]  
  • Toleman, M. A., Simm, A. M., Murphy, T. A., Gales, A. C., Biedenbach, D. J., Jones, R. N., Walsh, T. R. (2002). Molecular characterization of SPM-1, a novel metallo-{beta}-lactamase isolated in Latin America: report from the SENTRY antimicrobial surveillance programme. J Antimicrob Chemother 50: 673-679 [Abstract] [Full Text]  
  • Bellais, S., Girlich, D., Karim, A., Nordmann, P. (2002). EBR-1, a Novel Ambler Subclass B1 {beta}-Lactamase from Empedobacter brevis. Antimicrob. Agents Chemother. 46: 3223-3227 [Abstract] [Full Text]  
  • Bellais, S., Naas, T., Nordmann, P. (2002). Genetic and Biochemical Characterization of CGB-1, an Ambler Class B Carbapenem-Hydrolyzing {beta}-Lactamase from Chryseobacterium gleum. Antimicrob. Agents Chemother. 46: 2791-2796 [Abstract] [Full Text]  
  • Simm, A. M., Higgins, C. S., Carenbauer, A. L., Crowder, M. W., Bateson, J. H., Bennett, P. M., Clarke, A. R., Halford, S. E., Walsh, T. R. (2002). Characterization of Monomeric L1 Metallo-beta -lactamase and the Role of the N-terminal Extension in Negative Cooperativity and Antibiotic Hydrolysis. J. Biol. Chem. 277: 24744-24752 [Abstract] [Full Text]  
  • Mercuri, P. S., Bouillenne, F., Boschi, L., Lamotte-Brasseur, J., Amicosante, G., Devreese, B., van Beeumen, J., Frère, J.-M., Rossolini, G. M., Galleni, M. (2001). Biochemical Characterization of the FEZ-1 Metallo-{beta}-Lactamase of Legionella gormanii ATCC 33297T Produced in Escherichia coli. Antimicrob. Agents Chemother. 45: 1254-1262 [Abstract] [Full Text]  
  • Galleni, M., Lamotte-Brasseur, J., Rossolini, G. M., Spencer, J., Dideberg, O., Frère, J.-M. (2001). Standard Numbering Scheme for Class B {beta}-Lactamases. Antimicrob. Agents Chemother. 45: 660-663 [Full Text]  
  • Rossolini, G. M., Condemi, M. A., Pantanella, F., Docquier, J.-D., Amicosante, G., Thaller, M. C. (2001). Metallo-{beta}-Lactamase Producers in Environmental Microbiota: New Molecular Class B Enzyme in Janthinobacterium lividum. Antimicrob. Agents Chemother. 45: 837-844 [Abstract] [Full Text]  
  • Avison, M. B., Niumsup, P., Walsh, T. R., Bennett, P. M. (2000). Aeromonas hydrophila AmpH and CepH {beta}-lactamases: derepressed expression in mutants of Escherichia coli lacking creB. J Antimicrob Chemother 46: 695-702 [Abstract] [Full Text]  
  • Bellais, S., Poirel, L., Leotard, S., Naas, T., Nordmann, P. (2000). Genetic Diversity of Carbapenem-Hydrolyzing Metallo-beta -Lactamases from Chryseobacterium (Flavobacterium) indologenes. Antimicrob. Agents Chemother. 44: 3028-3034 [Abstract] [Full Text]  
  • Bonnet, R., Sampaio, J. L. M., Chanal, C., Sirot, D., De Champs, C., Viallard, J. L., Labia, R., Sirot, J. (2000). A Novel Class A Extended-Spectrum beta -Lactamase (BES-1) in Serratia marcescens Isolated in Brazil. Antimicrob. Agents Chemother. 44: 3061-3068 [Abstract] [Full Text]  
  • Haruta, S., Yamaguchi, H., Yamamoto, E. T., Eriguchi, Y., Nukaga, M., O'Hara, K., Sawai, T. (2000). Functional Analysis of the Active Site of a Metallo-beta -Lactamase Proliferating in Japan. Antimicrob. Agents Chemother. 44: 2304-2309 [Abstract] [Full Text]  
  • Bellais, S., Aubert, D., Naas, T., Nordmann, P. (2000). Molecular and Biochemical Heterogeneity of Class B Carbapenem-Hydrolyzing beta -Lactamases in Chryseobacterium meningosepticum. Antimicrob. Agents Chemother. 44: 1878-1886 [Abstract] [Full Text]  
  • Boschi, L., Mercuri, P. S., Riccio, M. L., Amicosante, G., Galleni, M., Frère, J.-M., Rossolini, G. M. (2000). The Legionella (Fluoribacter) gormanii Metallo-beta -Lactamase: a New Member of the Highly Divergent Lineage of Molecular-Subclass B3 beta -Lactamases. Antimicrob. Agents Chemother. 44: 1538-1543 [Abstract] [Full Text]  
  • Poirel, L., Naas, T., Nicolas, D., Collet, L., Bellais, S., Cavallo, J.-D., Nordmann, P. (2000). Characterization of VIM-2, a Carbapenem-Hydrolyzing Metallo-beta -Lactamase and Its Plasmid- and Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in France. Antimicrob. Agents Chemother. 44: 891-897 [Abstract] [Full Text]  
  • Poirel, L., Le Thomas, I., Naas, T., Karim, A., Nordmann, P. (2000). Biochemical Sequence Analyses of GES-1, a Novel Class A Extended-Spectrum beta -Lactamase, and the Class 1 Integron In52 from Klebsiella pneumoniae. Antimicrob. Agents Chemother. 44: 622-632 [Abstract] [Full Text]  
  • Bellais, S., Poirel, L., Naas, T., Girlich, D., Nordmann, P. (2000). Genetic-Biochemical Analysis and Distribution of the Ambler Class A beta -Lactamase CME-2, Responsible for Extended-Spectrum Cephalosporin Resistance in Chryseobacterium (Flavobacterium) meningosepticum. Antimicrob. Agents Chemother. 44: 1-9 [Abstract] [Full Text]  
  • Rossolini, G. M., Franceschini, N., Lauretti, L., Caravelli, B., Riccio, M. L., Galleni, M., Frère, J.-M., Amicosante, G. (1999). Cloning of a Chryseobacterium (Flavobacterium) meningosepticum Chromosomal Gene (blaACME) Encoding an Extended-Spectrum Class A beta -Lactamase Related to the Bacteroides Cephalosporinases and the VEB-1 and PER beta -Lactamases. Antimicrob. Agents Chemother. 43: 2193-2199 [Abstract] [Full Text]  
  • Lauretti, L., Riccio, M. L., Mazzariol, A., Cornaglia, G., Amicosante, G., Fontana, R., Rossolini, G. M. (1999). Cloning and Characterization of blaVIM, a New Integron-Borne Metallo-beta -Lactamase Gene from a Pseudomonas aeruginosa Clinical Isolate. Antimicrob. Agents Chemother. 43: 1584-1590 [Abstract] [Full Text]  
  • Laurent, F., Poirel, L., Naas, T., Chaibi, E. B., Labia, R., Boiron, P., Nordmann, P. (1999). Biochemical-Genetic Analysis and Distribution of FAR-1, a Class A beta -Lactamase from Nocardia farcinica. Antimicrob. Agents Chemother. 43: 1644-1650 [Abstract] [Full Text]  
  • Laraki, N., Franceschini, N., Rossolini, G. M., Santucci, P., Meunier, C., de Pauw, E., Amicosante, G., Frère, J. M., Galleni, M. (1999). Biochemical Characterization of the Pseudomonas aeruginosa 101/1477 Metallo-beta -Lactamase IMP-1 Produced by Escherichia coli. Antimicrob. Agents Chemother. 43: 902-906 [Abstract] [Full Text]  
  • Wang, Z., Benkovic, S. J. (1998). Purification, Characterization, and Kinetic Studies of a Soluble Bacteroides fragilis Metallo-beta -lactamase That Provides Multiple Antibiotic Resistance. J. Biol. Chem. 273: 22402-22408 [Abstract] [Full Text]  
  • Hirakata, Y., Izumikawa, K., Yamaguchi, T., Takemura, H., Tanaka, H., Yoshida, R., Matsuda, J., Nakano, M., Tomono, K., Maesaki, S., Kaku, M., Yamada, Y., Kamihira, S., Kohno, S. (1998). Rapid Detection and Evaluation of Clinical Characteristics of Emerging Multiple-Drug-Resistant Gram-Negative Rods Carrying the Metallo-beta -Lactamase Gene blaIMP. Antimicrob. Agents Chemother. 42: 2006-2011 [Abstract] [Full Text]  
  • Naas, T., Sougakoff, W., Casetta, A., Nordmann, P. (1998). Molecular Characterization of OXA-20, a Novel Class D beta -Lactamase, and Its Integron from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 42: 2074-2083 [Abstract] [Full Text]  
  • Sanschagrin, F., Dufresne, J., Levesque, R. C. (1998). Molecular Heterogeneity of the L-1 Metallo-beta -Lactamase Family from Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 42: 1245-1248 [Abstract] [Full Text]  
  • Walsh, T. R., Neville, W. A., Haran, M. H., Tolson, D., Payne, D. J., Bateson, J. H., Macgowan, A. P., Bennett, P. M. (1998). Nucleotide and Amino Acid Sequences of the Metallo-beta -Lactamase, ImiS, from Aeromonas veronii bv. sobria. Antimicrob. Agents Chemother. 42: 436-439 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»