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J Bacteriol. 1991 September; 173(17): 5558-5563

research-article

Stimulation of Agrobacterium tumefaciens T-DNA transfer by overdrive depends on a flanking sequence but not on helical position with respect to the border repeat.

C E Shurvinton and W Ream

Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-6502.

ABSTRACT

T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.

J Bacteriol. 1991 September; 173(17): 5558-5563




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