This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Redfield, R J Search for Related Content PubMed PubMed Citation Articles by Redfield, R J

 Previous Article  |  Next Article 

J Bacteriol. 1991 September; 173(18): 5612-5618

research-article

sxy-1, a Haemophilus influenzae mutation causing greatly enhanced spontaneous competence.

R J Redfield

Department of Biochemistry, University of British Columbia, Vancouver, Canada.

ABSTRACT

A Haemophilus influenzae strain carrying a competence-enhancing mutation (sxy-1) was selected by transformation of a mutagenized culture in exponential growth at low cell density, where spontaneous competence is very rare. Under these conditions, sxy-1 cells spontaneously transformed 100 to 1,000 times more efficiently than wild-type cells. Moreover, sxy-1 cells responded to all known competence-inducing treatments with further increases in transformation frequency. At high cell densities, sxy-1 cells spontaneously developed the level of competence reached by wild-type cells only after maximal induction by transfer to starvation medium. The sxy-1 mutation appears to act early in the sequence of events leading to competence; it increased the competence of cells carrying the early-acting transformation-defective (Tfo-) mutation tfo-98 by as large a factor as it did the competence of wild-type cells, but it had no effect when combined with another early-acting Tfo- mutation (tfo-87) or with the late-acting Tfo- mutation rec-2.

---

J Bacteriol. 1991 September; 173(18): 5612-5618

This article has been cited by other articles:

• Sinha, S., Cameron, A. D. S., Redfield, R. J. (2009). Sxy Induces a CRP-S Regulon in Escherichia coli. J. Bacteriol. 191: 5180-5195 [Abstract] [Full Text]  
• Cameron, A. D.S., Volar, M., Bannister, L. A., Redfield, R. J. (2008). RNA secondary structure regulates the translation of sxy and competence development in Haemophilus influenzae. Nucleic Acids Res 36: 10-20 [Abstract] [Full Text]  
• Merdanovic, M., Sauer, E., Reidl, J. (2005). Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae. J. Bacteriol. 187: 4410-4420 [Abstract] [Full Text]  
• VanWagoner, T. M., Whitby, P. W., Morton, D. J., Seale, T. W., Stull, T. L. (2004). Characterization of Three New Competence-Regulated Operons in Haemophilus influenzae. J. Bacteriol. 186: 6409-6421 [Abstract] [Full Text]  
• Ma, C., Redfield, R. J. (2000). Point Mutations in a Peptidoglycan Biosynthesis Gene Cause Competence Induction in Haemophilus influenzae. J. Bacteriol. 182: 3323-3330 [Abstract] [Full Text]  
• Gwinn, M. L., Ramanathan, R., Smith, H. O., Tomb, J.-F. (1998). A New Transformation-Deficient Mutant of Haemophilus influenzae Rd with Normal DNA Uptake. J. Bacteriol. 180: 746-748 [Abstract] [Full Text]  
• Fleischmann, R., Adams, M., White, O, Clayton, R., Kirkness, E., Kerlavage, A., Bult, C., Tomb, J., Dougherty, B., Merrick, J., al., e. (1995). Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269: 496-512 [Abstract]