This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moran, A P Articles by Zähringer, U Search for Related Content PubMed PubMed Citation Articles by Moran, A P Articles by Zähringer, U

 Previous Article  |  Next Article 

J Bacteriol. 1991 January; 173(2): 618-626

research-article

Chemical characterization of Campylobacter jejuni lipopolysaccharides containing N-acetylneuraminic acid and 2,3-diamino-2,3-dideoxy-D-glucose.

A P Moran, E T Rietschel, T U Kosunen and U Zähringer

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.

ABSTRACT

Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (H. Brade and C. Galanos, Eur. J. Biochem. 122:233-237, 1982) gave better yields of LPS than did extraction by the conventional hot phenol-water technique. Constituents common to all LPS were D-glucose, D-galactose, L-glycero-D-manno-heptose, 3-deoxy-D-manno-2-octulosonic acid, D-glucuronic acid, D-galactosamine, and phosphorylethanolamine. Phosphate was present in a relatively high amount. In addition, the LPS of three strains contained N-acetylneuraminic acid, whereas the LPS of the strain lacking this component contained 3-amino-3,6-dideoxy-D-glucose. The lipid A component contained phosphate with D-glucosamine and 2,3-diamino-2,3-dideoxy-D-glucose as the major amino sugars. Ethanolamine-phosphate was present also. The major fatty acids were ester- and amide-bound 3-hydroxytetradecanoic and ester-bound hexadecanoic acids, with a minor amount of ester-bound tetradecanoic acid. This is the first report of N-acetylneuraminic acid in the oligosaccharide moiety and diaminoglucose in the lipid A of C. jejuni LPS.

---

J Bacteriol. 1991 January; 173(2): 618-626

This article has been cited by other articles:

• Lewis, A. L., Hensler, M. E., Varki, A., Nizet, V. (2006). The Group B Streptococcal Sialic Acid O-Acetyltransferase Is Encoded by neuD, a Conserved Component of Bacterial Sialic Acid Biosynthetic Gene Clusters. J. Biol. Chem. 281: 11186-11192 [Abstract] [Full Text]  
• Prendergast, M. M., Tribble, D. R., Baqar, S., Scott, D. A., Ferris, J. A., Walker, R. I., Moran, A. P. (2004). In Vivo Phase Variation and Serologic Response to Lipooligosaccharide of Campylobacter jejuni in Experimental Human Infection. Infect. Immun. 72: 916-922 [Abstract] [Full Text]  
• Jones, P. A., Samuels, N. M., Phillips, N. J., Munson, R. S. Jr., Bozue, J. A., Arseneau, J. A., Nichols, W. A., Zaleski, A., Gibson, B. W., Apicella, M. A. (2002). Haemophilus influenzae Type b Strain A2 Has Multiple Sialyltransferases Involved in Lipooligosaccharide Sialylation. J. Biol. Chem. 277: 14598-14611 [Abstract] [Full Text]  
• Oldfield, N. J., Moran, A. P., Millar, L. A., Prendergast, M. M., Ketley, J. M. (2002). Characterization of the Campylobacter jejuni Heptosyltransferase II Gene, waaF, Provides Genetic Evidence that Extracellular Polysaccharide Is Lipid A Core Independent. J. Bacteriol. 184: 2100-2107 [Abstract] [Full Text]  
• Guerry, P., Szymanski, C. M., Prendergast, M. M., Hickey, T. E., Ewing, C. P., Pattarini, D. L., Moran, A. P. (2002). Phase Variation of Campylobacter jejuni 81-176 Lipooligosaccharide Affects Ganglioside Mimicry and Invasiveness In Vitro. Infect. Immun. 70: 787-793 [Abstract] [Full Text]  
• Ang, C. W., De Klerk, M. A., Endtz, H. P., Jacobs, B. C., Laman, J. D., van der Meche, F. G. A., van Doorn, P. A. (2001). Guillain-Barre Syndrome- and Miller Fisher Syndrome-Associated Campylobacter jejuni Lipopolysaccharides Induce Anti-GM1 and Anti-GQ1b Antibodies in Rabbits. Infect. Immun. 69: 2462-2469 [Abstract] [Full Text]  
• Prendergast, M. M., Kosunen, T. U., Moran, A. P. (2001). Development of an Immunoassay for Rapid Detection of Ganglioside GM1 Mimicry in Campylobacter jejuni Strains. J. Clin. Microbiol. 39: 1494-1500 [Abstract] [Full Text]  
• Gronow, S., Brade, H. (2001). Invited review: Lipopolysaccharide biosynthesis: which steps do bacteria need to survive?. Innate Immunity 7: 3-23 [Abstract]  
• Guerry, P., Ewing, C. P., Hickey, T. E., Prendergast, M. M., Moran, A. P. (2000). Sialylation of Lipooligosaccharide Cores Affects Immunogenicity and Serum Resistance of Campylobacter jejuni. Infect. Immun. 68: 6656-6662 [Abstract] [Full Text]  
• Prendergast, M. M., Moran, A. P. (2000). Review: Lipopolysaccharides in the development of the Guillain-Barre syndrome and Miller Fisher syndrome forms of acute inflammatory peripheral neuropathies. Innate Immunity 6: 341-359 [Abstract]  
• Prendergast, M. M., Willison, H. J., Moran, A. P. (1999). Human Monoclonal Immunoglobulin M Antibodies to Ganglioside GM1 Show Diverse Cross-Reactivities with Lipopolysaccharides of Campylobacter jejuni Strains Associated with Guillain-Barre Syndrome. Infect. Immun. 67: 3698-3701 [Abstract] [Full Text]  
• Prendergast, M. M., Lastovica, A. J., Moran, A. P. (1998). Lipopolysaccharides from Campylobacter jejuni O:41 Strains Associated with Guillain-Barre Syndrome Exhibit Mimicry of GM1 Ganglioside. Infect. Immun. 66: 3649-3655 [Abstract] [Full Text]  
• Schwerer, B., Neisser, A., Polt, R.J., Bernheimer, H., Moran, A.P. (1995). Antibody cross-reactivities between gangliosides and lipopolysaccharides of Campylobacter jejuni serotypes associated with Guillain-Barre syndrome. Innate Immunity 2: 395-403 [Abstract]  
• Pece, S., Fumarola, D., Giuliani, G., Jirillo, E., Moran, A.P. (1995). Activity in the Limulus amebocyte lysate assay and induction of tumor necrosis factor-{alpha} by diverse Helicobacter pylori lipopolysaccharide preparations. Innate Immunity 2: 455-462 [Abstract]