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16S ribosomal DNA amplification for phylogenetic study.

GENE-TRAK Systems, Framingham, Massachusetts 01701.

ABSTRACT

A set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. One pair of primers is capable of amplifying nearly full-length 16S ribosomal DNA (rDNA) from many bacterial genera; the additional primers are useful for various exceptional sequences. Methods for purification of amplified material, direct sequencing, cloning, sequencing, and transcription are outlined. An obligate intracellular parasite of bovine erythrocytes, Anaplasma marginale, is used as an example; its 16S rDNA was amplified, cloned, sequenced, and phylogenetically placed. Anaplasmas are related to the genera Rickettsia and Ehrlichia. In addition, 16S rDNAs from several species were readily amplified from material found in lyophilized ampoules from the American Type Culture Collection. By use of this method, the phylogenetic study of extremely fastidious or highly pathogenic bacterial species can be carried out without the need to culture them. In theory, any gene segment for which polymerase chain reaction primer design is possible can be derived from a readily obtainable lyophilized bacterial culture.

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• Vrijenhoek, R. C., Duhaime, M., Jones, W. J. (2007). Subtype Variation Among Bacterial Endosymbionts of Tubeworms (Annelida: Siboglinidae) from the Gulf of California. Biol. Bull. 212: 180-184 [Full Text]  
• Yoon, J., Yasumoto-Hirose, M., Katsuta, A., Sekiguchi, H., Matsuda, S., Kasai, H., Yokota, A. (2007). Coraliomargarita akajimensis gen. nov., sp. nov., a novel member of the phylum 'Verrucomicrobia' isolated from seawater in Japan. Int. J. Syst. Evol. Microbiol. 57: 959-963 [Abstract] [Full Text]  
• Yoon, J., Ishikawa, S., Kasai, H., Yokota, A. (2007). Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family 'Flammeovirgaceae' isolated from sediment. Int. J. Syst. Evol. Microbiol. 57: 964-968 [Abstract] [Full Text]  
• Yoon, J., Ishikawa, S., Kasai, H., Yokota, A. (2007). Persicitalea jodogahamensis gen. nov., sp. nov., a marine bacterium of the family 'Flexibacteraceae', isolated from seawater in Japan. Int. J. Syst. Evol. Microbiol. 57: 1014-1017 [Abstract] [Full Text]  
• Cui, Y.-S., Im, W.-T., Yin, C.-R., Lee, J.-S., Lee, K. C., Lee, S.-T. (2007). Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int. J. Syst. Evol. Microbiol. 57: 687-691 [Abstract] [Full Text]  
• Glazunova, O. O., Launay, T., Raoult, D., Roux, V. (2007). Prevotella timonensis sp. nov., isolated from a human breast abscess. Int. J. Syst. Evol. Microbiol. 57: 883-886 [Abstract] [Full Text]  
• Helsel, L. O., Hollis, D., Steigerwalt, A. G., Morey, R. E., Jordan, J., Aye, T., Radosevic, J., Jannat-Khah, D., Thiry, D., Lonsway, D. R., Patel, J. B., Daneshvar, M. I., Levett, P. N. (2007). Identification of "Haematobacter," a New Genus of Aerobic Gram-Negative Rods Isolated from Clinical Specimens, and Reclassification of Rhodobacter massiliensis as "Haematobacter massiliensis comb. nov.". J. Clin. Microbiol. 45: 1238-1243 [Abstract] [Full Text]  
• Weon, H.-Y., Kim, B.-Y., Kim, M.-K., Yoo, S.-H., Kwon, S.-W., Go, S.-J., Stackebrandt, E. (2007). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. Int. J. Syst. Evol. Microbiol. 57: 548-551 [Abstract] [Full Text]  
• Hatamoto, M., Imachi, H., Ohashi, A., Harada, H. (2007). Identification and Cultivation of Anaerobic, Syntrophic Long-Chain Fatty Acid-Degrading Microbes from Mesophilic and Thermophilic Methanogenic Sludges. Appl. Environ. Microbiol. 73: 1332-1340 [Abstract] [Full Text]  
• Madhaiyan, M., Kim, B.-Y., Poonguzhali, S., Kwon, S.-W., Song, M.-H., Ryu, J.-H., Go, S.-J., Koo, B.-S., Sa, T.-M. (2007). Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int. J. Syst. Evol. Microbiol. 57: 326-331 [Abstract] [Full Text]  
• Satomi, M., Vogel, B. F., Venkateswaran, K., Gram, L. (2007). Description of Shewanella glacialipiscicola sp. nov. and Shewanella algidipiscicola sp. nov., isolated from marine fish of the Danish Baltic Sea, and proposal that Shewanella affinis is a later heterotypic synonym of Shewanella colwelliana. Int. J. Syst. Evol. Microbiol. 57: 347-352 [Abstract] [Full Text]  
• Dutta, D., Gachhui, R. (2007). Nitrogen-fixing and cellulose-producing Gluconacetobacter kombuchae sp. nov., isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 57: 353-357 [Abstract] [Full Text]  
• Vilchez, R., Pozo, C., Gomez, M. A., Rodelas, B., Gonzalez-Lopez, J. (2007). Dominance of sphingomonads in a copper-exposed biofilm community for groundwater treatment. Microbiology 153: 325-337 [Abstract] [Full Text]  
• Okabe, S., Odagiri, M., Ito, T., Satoh, H. (2007). Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems. Appl. Environ. Microbiol. 73: 971-980 [Abstract] [Full Text]  
• Brown, C. J., Wong, M., Davis, C. C., Kanti, A., Zhou, X., Forney, L. J. (2007). Preliminary characterization of the normal microbiota of the human vulva using cultivation-independent methods. J Med Microbiol 56: 271-276 [Abstract] [Full Text]  
• Tofteland, S., Haldorsen, B., Dahl, K. H., Simonsen, G. S., Steinbakk, M., Walsh, T. R., Sundsfjord, A., the Norwegian ESBL Study Group, (2007). Effects of Phenotype and Genotype on Methods for Detection of Extended-Spectrum-{beta}-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Norway. J. Clin. Microbiol. 45: 199-205 [Abstract] [Full Text]  
• He, Z., Kisla, D., Zhang, L., Yuan, C., Green-Church, K. B., Yousef, A. E. (2007). Isolation and Identification of a Paenibacillus polymyxa Strain That Coproduces a Novel Lantibiotic and Polymyxin. Appl. Environ. Microbiol. 73: 168-178 [Abstract] [Full Text]  
• Pankratov, T. A., Kulichevskaya, I. S., Liesack, W., Dedysh, S. N. (2006). Isolation of aerobic, gliding, xylanolytic and laminarinolytic bacteria from acidic Sphagnum peatlands and emended description of Chitinophaga arvensicola Kampfer et al. 2006. Int. J. Syst. Evol. Microbiol. 56: 2761-2764 [Abstract] [Full Text]  
• Matsuo, Y., Katsuta, A., Matsuda, S., Shizuri, Y., Yokota, A., Kasai, H. (2006). Mechercharimyces mesophilus gen. nov., sp. nov. and Mechercharimyces asporophorigenens sp. nov., antitumour substance-producing marine bacteria, and description of Thermoactinomycetaceae fam. nov.. Int. J. Syst. Evol. Microbiol. 56: 2837-2842 [Abstract] [Full Text]  
• Yang, S.-H., Kwon, K. K., Lee, H.-S., Kim, S.-J. (2006). Shewanella spongiae sp. nov., isolated from a marine sponge. Int. J. Syst. Evol. Microbiol. 56: 2879-2882 [Abstract] [Full Text]  
• Geider, K., Auling, G., Du, Z., Jakovljevic, V., Jock, S., Volksch, B. (2006). Erwinia tasmaniensis sp. nov., a non-phytopathogenic bacterium from apple and pear trees. Int. J. Syst. Evol. Microbiol. 56: 2937-2943 [Abstract] [Full Text]