This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gormley, E P Articles by Davies, J Search for Related Content PubMed PubMed Citation Articles by Gormley, E P Articles by Davies, J

 Previous Article  |  Next Article 

J Bacteriol. 1991 November; 173(21): 6705-6708

research-article

Transfer of plasmid RSF1010 by conjugation from Escherichia coli to Streptomyces lividans and Mycobacterium smegmatis.

E P Gormley and J Davies

Unité de Genié Microbiologique, Institut Pasteur, Paris, France.

ABSTRACT

The plasmid RSF1010 belongs to a class of plasmids (IncQ) that replicate in a range of bacterial hosts. Although non-self-transmissible, it can be mobilized at high frequency between different gram-negative bacterial species if transfer functions are supplied in trans. We report the transfer of RSF1010 by conjugation from Escherichia coli to the gram-positive actinomycetes Streptomyces lividans and Mycobacterium smegmatis. In its new hosts, the plasmid was stable with respect to structure and inheritance and conferred high-level resistance to streptomycin and sulfonamide. This is the first reported case of conjugative transfer of a naturally occurring plasmid between gram-negative and gram-positive bacteria.

---

J Bacteriol. 1991 November; 173(21): 6705-6708

This article has been cited by other articles:

• Ramon-Garcia, S., Otal, I., Martin, C., Gomez-Lus, R., Ainsa, J. A. (2006). Novel Streptomycin Resistance Gene from Mycobacterium fortuitum. Antimicrob. Agents Chemother. 50: 3920-3922 [Abstract] [Full Text]  
• Krzywinska, E., Krzywinski, J., Schorey, J. S. (2004). Naturally occurring horizontal gene transfer and homologous recombination in Mycobacterium. Microbiology 150: 1707-1712 [Abstract] [Full Text]  
• Rawlings, D. E., Tietze, E. (2001). Comparative Biology of IncQ and IncQ-Like Plasmids. Microbiol. Mol. Biol. Rev. 65: 481-496 [Abstract] [Full Text]  
• Su, H., Shao, Z., Tkalec, L., Blain, F., Zimmermann, J. (2001). Development of a genetic system for the transfer of DNA into Flavobacterium heparinum. Microbiology 147: 581-589 [Abstract] [Full Text]  
• Smalla, K., Heuer, H., Götz, A., Niemeyer, D., Krögerrecklenfort, E., Tietze, E. (2000). Exogenous Isolation of Antibiotic Resistance Plasmids from Piggery Manure Slurries Reveals a High Prevalence and Diversity of IncQ-Like Plasmids. Appl. Environ. Microbiol. 66: 4854-4862 [Abstract] [Full Text]  
• Scherzinger, E., Ziegelin, G., Barcena, M., Carazo, J. M., Lurz, R., Lanka, E. (1997). The RepA Protein of Plasmid RSF1010 Is a Replicative DNA Helicase. J. Biol. Chem. 272: 30228-30236 [Abstract] [Full Text]  
• Davies, J (1994). Inactivation of antibiotics and the dissemination of resistance genes. Science 264: 375-382 [Abstract]