This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuroda, A
Right arrow Articles by Sekiguchi, J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuroda, A
Right arrow Articles by Sekiguchi, J

 Previous Article  |  Next Article 

J Bacteriol. 1991 November; 173(22): 7304-7312

research-article

Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene.

A Kuroda and J Sekiguchi

Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

ABSTRACT

A major Bacillus subtilis 168S autolysin (N-acetylmuramoyl-L-alanine amidase [EC 3.5.1.28]) was purified and then cleaved with cyanogen bromide. The N-terminal amino acid sequence of one of the resultant peptides was determined in order to make synthetic oligonucleotides. A 2.5-kb EcoRI fragment was cloned into Escherichia coli JM109 and detected by colony hybridization by using the oligonucleotides as probes. Sequencing of the insert showed the presence of an open reading frame (designated cwlB), starting at a UUG codon, which encodes a polypeptide of 496 amino acids with a molecular mass of 52,623 Da. CWLB had a presumed signal peptide which is processed after Ala at position 24. Insertional inactivation of the cwlB gene of the B. subtilis chromosome led to an approximately 90% decrease in the total cell wall hydrolytic activity of stationary-phase cells and extraordinary resistance to cell lysis, even after 6 days of incubation at 37 degrees C. No apparent changes in cell morphology, motility, competence, sporulation, or germination were observed.

J Bacteriol. 1991 November; 173(22): 7304-7312




This article has been cited by other articles:

  • Chen, R., Guttenplan, S. B., Blair, K. M., Kearns, D. B. (2009). Role of the {sigma}D-Dependent Autolysins in Bacillus subtilis Population Heterogeneity. J. Bacteriol. 191: 5775-5784 [Abstract] [Full Text]  
  • Ahn, S.-J., Burne, R. A. (2006). The atlA Operon of Streptococcus mutans: Role in Autolysin Maturation and Cell Surface Biogenesis.. J. Bacteriol. 188: 6877-6888 [Abstract] [Full Text]  
  • Fukushima, T., Afkham, A., Kurosawa, S.-i., Tanabe, T., Yamamoto, H., Sekiguchi, J. (2006). A New D,L-Endopeptidase Gene Product, YojL (Renamed CwlS), Plays a Role in Cell Separation with LytE and LytF in Bacillus subtilis.. J. Bacteriol. 188: 5541-5550 [Abstract] [Full Text]  
  • Shibata, Y., Kawada, M., Nakano, Y., Toyoshima, K., Yamashita, Y. (2005). Identification and Characterization of an Autolysin-Encoding Gene of Streptococcus mutans. Infect. Immun. 73: 3512-3520 [Abstract] [Full Text]  
  • Yamamoto, H., Kurosawa, S.-i., Sekiguchi, J. (2003). Localization of the Vegetative Cell Wall Hydrolases LytC, LytE, and LytF on the Bacillus subtilis Cell Surface and Stability of These Enzymes to Cell Wall-Bound or Extracellular Proteases. J. Bacteriol. 185: 6666-6677 [Abstract] [Full Text]  
  • Yoshida, A., Kuramitsu, H. K. (2002). Multiple Streptococcus mutans Genes Are Involved in Biofilm Formation. Appl. Environ. Microbiol. 68: 6283-6291 [Abstract] [Full Text]  
  • Zimmer, M., Vukov, N., Scherer, S., Loessner, M. J. (2002). The Murein Hydrolase of the Bacteriophage {phi}3626 Dual Lysis System Is Active against All Tested Clostridium perfringens Strains. Appl. Environ. Microbiol. 68: 5311-5317 [Abstract] [Full Text]  
  • De Las Rivas, B., Garcia, J. L., Lopez, R., Garcia, P. (2002). Purification and Polar Localization of Pneumococcal LytB, a Putative Endo-{beta}-N-Acetylglucosaminidase: the Chain-Dispersing Murein Hydrolase. J. Bacteriol. 184: 4988-5000 [Abstract] [Full Text]  
  • Karjalainen, T., Waligora-Dupriet, A.-J., Cerquetti, M., Spigaglia, P., Maggioni, A., Mauri, P., Mastrantonio, P. (2001). Molecular and Genomic Analysis of Genes Encoding Surface-Anchored Proteins from Clostridium difficile. Infect. Immun. 69: 3442-3446 [Abstract] [Full Text]  
  • Waligora, A.-J., Hennequin, C., Mullany, P., Bourlioux, P., Collignon, A., Karjalainen, T. (2001). Characterization of a Cell Surface Protein of Clostridium difficile with Adhesive Properties. Infect. Immun. 69: 2144-2153 [Abstract] [Full Text]  
  • Meador-Parton, J., Popham, D. L. (2000). Structural Analysis of Bacillus subtilis Spore Peptidoglycan during Sporulation. J. Bacteriol. 182: 4491-4499 [Abstract] [Full Text]  
  • Smith, T. J., Blackman, S. A., Foster, S. J. (2000). Autolysins of Bacillus subtilis: multiple enzymes with multiple functions. Microbiology 146: 249-262 [Full Text]  
  • Nugroho, F. A., Yamamoto, H., Kobayashi, Y., Sekiguchi, J. (1999). Characterization of a New Sigma-K-Dependent Peptidoglycan Hydrolase Gene That Plays a Role in Bacillus subtilis Mother Cell Lysis. J. Bacteriol. 181: 6230-6237 [Abstract] [Full Text]  
  • Ohnishi, R., Ishikawa, S., Sekiguchi, J. (1999). Peptidoglycan Hydrolase LytF Plays a Role in Cell Separation with CwlF during Vegetative Growth of Bacillus subtilis. J. Bacteriol. 181: 3178-3184 [Abstract] [Full Text]  
  • Navarre, W. W., Schneewind, O. (1999). Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope. Microbiol. Mol. Biol. Rev. 63: 174-229 [Abstract] [Full Text]  
  • Stephenson, K., Harwood, C. R. (1998). Influence of a Cell-Wall-Associated Protease on Production of alpha -Amylase by Bacillus subtilis. Appl. Environ. Microbiol. 64: 2875-2881 [Abstract] [Full Text]  
  • Ishikawa, S., Hara, Y., Ohnishi, R., Sekiguchi, J. (1998). Regulation of a New Cell Wall Hydrolase Gene, cwlF, Which Affects Cell Separation in Bacillus subtilis. J. Bacteriol. 180: 2549-2555 [Abstract] [Full Text]  
  • Ishikawa, S., Yamane, K., Sekiguchi, J. (1998). Regulation and Characterization of a Newly Deduced Cell Wall Hydrolase Gene (cwlJ) Which Affects Germination of Bacillus subtilis Spores. J. Bacteriol. 180: 1375-1380 [Abstract] [Full Text]  
  • Popham, D. L., Helin, J., Costello, C. E., Setlow, P. (1996). Muramic lactam in peptidoglycan of Bacillus subtilis spores is required for spore outgrowth but not for spore dehydration or heat resistance. Proc. Natl. Acad. Sci. USA 93: 15405-15410 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»