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J Bacteriol. 1991 February; 173(4): 1554-1560

research-article

Characterization of the late-gene regulatory region of phage 21.

H C Guo, M Kainz and J W Roberts

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.

ABSTRACT

A segment of Escherichia coli bacteriophage 21 DNA encoding the late-gene regulator, Q21, and the late-gene leader RNA segment was sequenced; its structure is similar to those of the related phages lambda and 82. The leader RNA is about 45 nucleotides long and consists essentially entirely of sequences encoding the p-independent terminator that is the putative target of the antitermination activity of Q21. Like the corresponding regions of lambda and 82, the 21 late-gene promoter segment encodes an early transcription pause in vitro, at about nucleotide 18, during which Q21 presumably acts to modify RNA polymerase. The 21 Q gene, cloned in isolation, is active on the late-gene leader segment in trans, and its purified product is active as an antiterminator in vitro; Q21 represents a third late-gene antiterminator, in addition to those of lambda and 82. There is little evident similarity in the primary sequences of the three Q genes.

J Bacteriol. 1991 February; 173(4): 1554-1560




This article has been cited by other articles:

  • Santangelo, T. J., Mooney, R. A., Landick, R., Roberts, J. W. (2003). RNA polymerase mutations that impair conversion to a termination-resistant complex by Q antiterminator proteins. Genes Dev. 17: 1281-1292 [Abstract] [Full Text]  
  • Weisberg, R. A., Gottesman, M. E. (1999). Processive Antitermination. J. Bacteriol. 181: 359-367 [Full Text]  
  • Kainz, M, Roberts, J (1992). Structure of transcription elongation complexes in vivo. Science 255: 838-841 [Abstract]  


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