This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McCleary, W R
Right arrow Articles by Zusman, D R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCleary, W R
Right arrow Articles by Zusman, D R

 Previous Article

J Bacteriol. 1991 March; 173(6): 2141-2145

research-article

Myxococcus xanthus protein C is a major spore surface protein.

W R McCleary, B Esmon and D R Zusman

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

ABSTRACT

Fruiting body formation in Myxococcus xanthus involves the aggregation of cells to form mounds and the differentiation of rod-shaped cells into spherical myxospores. The surface of the myxospore is composed of several sodium dodecyl sulfate (SDS)-soluble proteins, the best characterized of which is protein S (Mr, 19,000). We have identified a new major spore surface protein called protein C (Mr, 30,000). Protein C is not present in extracts of vegetative cells but appears in extracts of developing cells by 6 h. Protein C, like protein S, is produced during starvation in liquid medium but is not made during glycerol-induced sporulation. Its synthesis is blocked in certain developmental mutants but not others. When examined by SDS-polyacrylamide gel electrophoresis, two forms of protein C are observed. Protein C is quantitatively released from spores by treatment with 0.1 N NaOH or by boiling in 1% SDS. It is slowly washed from the spore surface in water but is stabilized by the presence of magnesium. Protein C binds to the surface of spores depleted of protein C and protein S. Protein C is a useful new marker for development in M. xanthus because it is developmentally regulated, spore associated, abundant, and easily purified.

J Bacteriol. 1991 March; 173(6): 2141-2145




This article has been cited by other articles:

  • Kobayashi, H., Yoshida, T., Inouye, M. (2009). Significant Enhanced Expression and Solubility of Human Proteins in Escherichia coli by Fusion with Protein S from Myxococcus xanthus. Appl. Environ. Microbiol. 75: 5356-5362 [Abstract] [Full Text]  
  • Dahl, J. L., Tengra, F. K., Dutton, D., Yan, J., Andacht, T. M., Coyne, L., Windell, V., Garza, A. G. (2007). Identification of Major Sporulation Proteins of Myxococcus xanthus Using a Proteomic Approach. J. Bacteriol. 189: 3187-3197 [Abstract] [Full Text]  
  • Tengra, F. K., Dahl, J. L., Dutton, D., Caberoy, N. B., Coyne, L., Garza, A. G. (2006). CbgA, a Protein Involved in Cortex Formation and Stress Resistance in Myxococcus xanthus Spores. J. Bacteriol. 188: 8299-8302 [Abstract] [Full Text]  
  • Crawford, E. W. Jr., Shimkets, L. J. (2000). The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein. Genes Dev. 14: 483-492 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»