This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Allardet-Servent, A
Right arrow Articles by Ramuz, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Allardet-Servent, A
Right arrow Articles by Ramuz, M

 Previous Article  |  Next Article 

J Bacteriol. 1991 April; 173(7): 2219-2224

research-article

Physical map of the Brucella melitensis 16 M chromosome.

A Allardet-Servent, M J Carles-Nurit, G Bourg, S Michaux and M Ramuz

Faculté de Médecine, Unité 65 Montpellier-Nîmes, Institut National de la Santé et de la Recherche Médicale, France.

ABSTRACT

We present the first restriction map of the Brucella melitensis 16 M chromosome obtained by Southern blot hybridization of SpeI, XhoI, and XbaI fragments separated by pulsed-field gel electrophoresis. All restriction fragments (a total of 113) were mapped into an open circle. The main difficulty in mapping involved the exceedingly high number of restriction fragments, as was expected considering the 59% G + C content of the Brucella genome. Several cloned genes were placed on this map, especially rRNA operons which are repeated three times. The size of the B. melitensis chromosome, estimated as 2,600 kb long in a previous study, appeared longer (3,130 kb) by restriction mapping. This restriction map is an initial approach to achieve a genetic map of the Brucella chromosome.

J Bacteriol. 1991 April; 173(7): 2219-2224






Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»