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J Bacteriol. 1992 May; 174(10): 3399-3402

research-article

Dependence of oxygen-tolerant nitrogenase activity on divalent cations in Azotobacter vinelandii.

J B Peterson

Department of Botany, Iowa State University, Ames 50011.

ABSTRACT

Nitrogenase activity of washed Azotobacter vinelandii cells was enhanced by the addition of Ca2+ and Mg2+, and the enhancement increased with the O2 concentration. In assays provided with a level of O2 that was initially supraoptimal and inhibitory to nitrogenase activity, the addition of Ca2+ or Mg2+ affected both the maximum respiration rate (Vmax) of the cells and the apparent affinity [KS(O2)] of cell respiration for O2. Changes in these parameters correlated with changes in nitrogenase activity. Aeration-dependent increases in Vmax and KS(O2) were inhibited by rifampin and chloramphenicol and were also observed in ammonium-grown cultures.

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J Bacteriol. 1992 May; 174(10): 3399-3402

This article has been cited by other articles:

• Meek, L., Arp, D. J. (2000). The Hydrogenase Cytochrome b Heme Ligands of Azotobacter vinelandii Are Required for Full H2 Oxidation Capability. J. Bacteriol. 182: 3429-3436 [Abstract] [Full Text]