This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barsomian, G D Articles by Walker, G C Search for Related Content PubMed PubMed Citation Articles by Barsomian, G D Articles by Walker, G C

 Previous Article  |  Next Article 

J Bacteriol. 1992 July; 174(13): 4416-4426

research-article

Rhizobium meliloti mutants unable to synthesize anthranilate display a novel symbiotic phenotype.

G D Barsomian, A Urzainqui, K Lohman and G C Walker

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

ABSTRACT

Analyses of Rhizobium meliloti trp auxotrophs suggest that anthranilate biosynthesis by the R. meliloti trpE(G) gene product is necessary during nodule development for establishment of an effective symbiosis. trpE(G) mutants, as well as mutants blocked earlier along this pathway in aromatic amino acid biosynthesis, form nodules on alfalfa that have novel defects. In contrast, R. meliloti trp mutants blocked later in the tryptophan-biosynthetic pathway form normal, pink, nitrogen-fixing nodules. trpE(G) mutants form two types of elongated, defective nodules containing unusually extended invasion zones on alfalfa. One type contains bacteroids in its base and is capable of nitrogen fixation, while the other lacks bacteroids and cannot fix nitrogen. The trpE(G) gene is expressed in normal nodules. Models are discussed to account for these observations, including one in which anthranilate is postulated to act as an in planta siderophore.

---

J Bacteriol. 1992 July; 174(13): 4416-4426

This article has been cited by other articles:

• Buendia-Claveria, A. M., Moussaid, A., Ollero, F. J., Vinardell, J. M., Torres, A., Moreno, J., Gil-Serrano, A. M., Rodriguez-Carvajal, M. A., Tejero-Mateo, P., Peart, J. L., Brewin, N. J., Ruiz-Sainz, J. E. (2003). A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology 149: 1807-1818 [Abstract] [Full Text]  
• Patriarca, E. J., Tate, R., Iaccarino, M. (2002). Key Role of Bacterial NH4+ Metabolism in Rhizobium-Plant Symbiosis. Microbiol. Mol. Biol. Rev. 66: 203-222 [Abstract] [Full Text]  
• Lynch, D., O'Brien, J., Welch, T., Clarke, P., Ó Cuív, P., Crosa, J. H., O'Connell, M. (2001). Genetic Organization of the Region Encoding Regulation, Biosynthesis, and Transport of Rhizobactin 1021, a Siderophore Produced by Sinorhizobium meliloti. J. Bacteriol. 183: 2576-2585 [Abstract] [Full Text]  
• Yeoman, K. H., Wisniewski-Dye, F., Timony, C., Stevens, J. B., deLuca, N. G., Downie, J. A., Johnston, A. W. B. (2000). Analysis of the Rhizobium leguminosarum siderophore-uptake gene fhuA: differential expression in free-living bacteria and nitrogen-fixing bacteroids and distribution of an fhuA pseudogene in different strains. Microbiology 146: 829-837 [Abstract] [Full Text]