This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaasen, I
Right arrow Articles by Strøm, A R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaasen, I
Right arrow Articles by Strøm, A R

 Previous Article  |  Next Article 

J Bacteriol. 1992 February; 174(3): 889-898

research-article

Molecular cloning and physical mapping of the otsBA genes, which encode the osmoregulatory trehalose pathway of Escherichia coli: evidence that transcription is activated by katF (AppR)

I Kaasen, P Falkenberg, O B Styrvold and A R Strøm

Norwegian College of Fishery Science, University of Tromsø, Norway.

ABSTRACT

It has been shown previously that the otsA and otsB mutations block osmoregulatory trehalose synthesis in Escherichia coli. We report that the transcription of these osmoregulated ots genes is dependent on KatF (AppR), a putative sigma factor for certain stationary phase- and starvation-induced genes. The transcription of the osmoregulated bet and proU genes was not katF dependent. Our genetic analysis showed that katF carries an amber mutation in E. coli K-12 and many of its derivatives but that katF has reverted to an active form in the much-used strain MC4100. This amber mutation in katF leads to strain variations in trehalose synthesis and other katF-dependent functions of E. coli. We have performed a molecular cloning of the otsBA genes, and we present evidence that they constitute an operon encoding trehalose-6-phosphate phosphatase and trehalose-6-phosphate synthase. A cloning and restriction site analysis, performed by comparing the cloned fragments with the known physical map of the E. coli chromosome, revealed that the otsBA genes are situated on a 2.9-kb HindIII fragment located 8 to 11 kb clockwise of tar (41.6 min).

J Bacteriol. 1992 February; 174(3): 889-898




This article has been cited by other articles:

  • Dominguez-Ferreras, A., Soto, M. J., Perez-Arnedo, R., Olivares, J., Sanjuan, J. (2009). Importance of Trehalose Biosynthesis for Sinorhizobium meliloti Osmotolerance and Nodulation of Alfalfa Roots. J. Bacteriol. 191: 7490-7499 [Abstract] [Full Text]  
  • Xu, J., Bao, B., Zhang, Z.-F., Yi, Y.-Z., Xu, W.-H. (2009). Identification of a novel gene encoding the trehalose phosphate synthase in the cotton bollworm, Helicoverpa armigera. Glycobiology 19: 250-257 [Abstract] [Full Text]  
  • Tondervik, A., Torgersen, H. R., Botnmark, H. K., Strom, A. R. (2006). Transposon Mutations in the 5' End of glnD, the Gene for a Nitrogen Regulatory Sensor, That Suppress the Osmosensitive Phenotype Caused by otsBA Lesions in Escherichia coli.. J. Bacteriol. 188: 4218-4226 [Abstract] [Full Text]  
  • Vijayakumar, S. R. V., Kirchhof, M. G., Patten, C. L., Schellhorn, H. E. (2004). RpoS-Regulated Genes of Escherichia coli Identified by Random lacZ Fusion Mutagenesis. J. Bacteriol. 186: 8499-8507 [Abstract] [Full Text]  
  • Goude, R., Renaud, S., Bonnassie, S., Bernard, T., Blanco, C. (2004). Glutamine, Glutamate, and {alpha}-Glucosylglycerate Are the Major Osmotic Solutes Accumulated by Erwinia chrysanthemi Strain 3937. Appl. Environ. Microbiol. 70: 6535-6541 [Abstract] [Full Text]  
  • Padilla, L., Morbach, S., Kramer, R., Agosin, E. (2004). Impact of Heterologous Expression of Escherichia coli UDP-Glucose Pyrophosphorylase on Trehalose and Glycogen Synthesis in Corynebacterium glutamicum. Appl. Environ. Microbiol. 70: 3845-3854 [Abstract] [Full Text]  
  • Padilla, L., Kramer, R., Stephanopoulos, G., Agosin, E. (2004). Overproduction of Trehalose: Heterologous Expression of Escherichia coli Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase in Corynebacterium glutamicum. Appl. Environ. Microbiol. 70: 370-376 [Abstract] [Full Text]  
  • Schluepmann, H., Pellny, T., van Dijken, A., Smeekens, S., Paul, M. (2003). Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 100: 6849-6854 [Abstract] [Full Text]  
  • Elbein, A. D., Pan, Y.T., Pastuszak, I., Carroll, D. (2003). New insights on trehalose: a multifunctional molecule. Glycobiology 13: 17R-27R [Abstract] [Full Text]  
  • Klutts, S., Pastuszak, I., Edavana, V. K., Thampi, P., Pan, Y.-T., Abraham, E. C., Carroll, J. D., Elbein, A. D. (2003). Purification, Cloning, Expression, and Properties of Mycobacterial Trehalose-phosphate Phosphatase. J. Biol. Chem. 278: 2093-2100 [Abstract] [Full Text]  
  • Kandror, O., DeLeon, A., Goldberg, A. L. (2002). Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures. Proc. Natl. Acad. Sci. USA 99: 9727-9732 [Abstract] [Full Text]  
  • Marin, K., Huckauf, J., Fulda, S., Hagemann, M. (2002). Salt-Dependent Expression of Glucosylglycerol-Phosphate Synthase, Involved in Osmolyte Synthesis in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J. Bacteriol. 184: 2870-2877 [Abstract] [Full Text]  
  • Notley-McRobb, L., King, T., Ferenci, T. (2002). rpoS Mutations and Loss of General Stress Resistance in Escherichia coli Populations as a Consequence of Conflict between Competing Stress Responses. J. Bacteriol. 184: 806-811 [Abstract] [Full Text]  
  • Haldimann, A., Wanner, B. L. (2001). Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria. J. Bacteriol. 183: 6384-6393 [Abstract] [Full Text]  
  • Cánovas, D., Fletcher, S. A., Hayashi, M., Csonka, L. N. (2001). Role of Trehalose in Growth at High Temperature of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 183: 3365-3371 [Abstract] [Full Text]  
  • Seo, H. S., Koo, Y. J., Lim, J. Y., Song, J. T., Kim, C. H., Kim, J. K., Lee, J. S., Choi, Y. D. (2000). Characterization of a Bifunctional Enzyme Fusion of Trehalose-6-Phosphate Synthetase and Trehalose-6-Phosphate Phosphatase of Escherichia coli. Appl. Environ. Microbiol. 66: 2484-2490 [Abstract] [Full Text]  
  • Andersson, R. A., Koiv, V., Norman-Setterblad, C., Pirhonen, M. (1999). Role of RpoS in virulence and stress tolerance of the plant pathogen Erwinia carotovora subsp. carotovora. Microbiology 145: 3547-3556 [Abstract] [Full Text]  
  • Bell, W., Sun, W., Hohmann, S., Wera, S., Reinders, A., De Virgilio, C., Wiemken, A., Thevelein, J. M. (1998). Composition and Functional Analysis of the Saccharomyces cerevisiae Trehalose Synthase Complex. J. Biol. Chem. 273: 33311-33319 [Abstract] [Full Text]  
  • Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
  • Germer, J., Muffler, A., Hengge-Aronis, R. (1998). Trehalose Is Not Relevant for In Vivo Activity of sigma S-Containing RNA Polymerase in Escherichia coli. J. Bacteriol. 180: 1603-1606 [Abstract] [Full Text]  
  • Boos, W., Shuman, H. (1998). Maltose/Maltodextrin System of Escherichia coli: Transport, Metabolism, and Regulation. Microbiol. Mol. Biol. Rev. 62: 204-229 [Abstract] [Full Text]  
  • Horlacher, R., Boos, W. (1997). Characterization of TreR, the Major Regulator of the Escherichia coli Trehalose System. J. Biol. Chem. 272: 13026-13032 [Abstract] [Full Text]  
  • Bishop, R. E., Penfold, S. S., Frost, L. S., Höltje, J.-V., Weiner, J. H. (1995). Stationary Phase Expression of a Novel Escherichia coli Outer Membrane Lipoprotein and Its Relationship with Mammalian Apolipoprotein D. J. Biol. Chem. 270: 23097-23103 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»