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J Bacteriol. 1992 February; 174(4): 1197-1204

research-article

Restriction endonuclease mapping of the HI2 incompatibility group plasmid R478.

Department of Microbiology, University College Galway, Ireland.

ABSTRACT

A restriction map of the 272-kb IncHI2 plasmid R478 was constructed by using the enzymes ApaI, XbaI, SalI, and XhoI. The map was derived from cloned restriction fragments from R478 inserted into cosmid and plasmid vectors as well as from double-digestion analysis of R478 and R478 miniplasmids. All previously known resistance determinants were cloned from R478, and their positions were located on the restriction map. A region involved in incompatibility was cloned and mapped. The location of a previously unreported arsenite resistance gene was also determined. The genes encoding tellurite resistance, colicin B resistance, and phage inhibition were found to be associated with a 6.7-kb SalI fragment of R478.

This article has been cited by other articles:

• Page, D. T., Whelan, K. F., Colleran, E. (2001). Characterization of two autoreplicative regions of the IncHI2 plasmid R478: RepHI2A and RepHI1A(R478). Microbiology 147: 1591-1598 [Abstract] [Full Text]