This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Woldringh, C L Articles by Vischer, N O Search for Related Content PubMed PubMed Citation Articles by Woldringh, C L Articles by Vischer, N O

 Previous Article  |  Next Article 

J Bacteriol. 1993 May; 175(10): 3174-3181

research-article

Volume growth of daughter and parent cells during the cell cycle of Saccharomyces cerevisiae a/alpha as determined by image cytometry.

C L Woldringh, P G Huls and N O Vischer

Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.

ABSTRACT

The pattern of volume growth of Saccharomyces cerevisiae a/alpha was determined by image cytometry for daughter cells and consecutive cycles of parent cells. An image analysis program was specially developed to measure separately the volume of bud and mother cell parts and to quantify the number of bud scars on each parent cell. All volumetric data and cell attributes (budding state, number of scars) were stored in such a way that separate volume distributions of cells or cell parts with any combination of properties--for instance, buds present on mothers with two scars or cells without scars (i.e., daughter cells) and without buds--could be obtained. By a new method called intersection analysis, the average volumes of daughter and parent cells at birth and at division could be determined for a steady-state population. These volumes compared well with those directly measured from cells synchronized by centrifugal elutriation. During synchronous growth of daughter cells, the pattern of volume increase appeared to be largely exponential. However, after bud emergence, larger volumes than those predicted by a continuous exponential increase were obtained, which confirms the reported decrease in buoyant density. The cycle times calculated from the steady-state population by applying the age distribution equation deviated from those directly obtained from the synchronized culture, probably because of inadequate scoring of bud scars. Therefore, for the construction of a volume-time diagram, we used volume measurements obtained from the steady-state population and cycle times obtained from the synchronized population. The diagram shows that after bud emergence, mother cell parts continue to grow at a smaller rate, increasing about 10% in volume during the budding period. Second-generation daughter cells, ie., cells born from parents left with two scars, were significantly smaller than first-generation daughter cells. Second- and third-generation parent cells showed a decreased volume growth rate and a shorter budding period than that of daughter cells.

---

J Bacteriol. 1993 May; 175(10): 3174-3181

This article has been cited by other articles:

• Goranov, A. I., Cook, M., Ricicova, M., Ben-Ari, G., Gonzalez, C., Hansen, C., Tyers, M., Amon, A. (2009). The rate of cell growth is governed by cell cycle stage. Genes Dev. 23: 1408-1422 [Abstract] [Full Text]  
• Sugiura, M., Georgescu, M. N., Takahashi, M. (2007). A Nitrite Transporter Associated with Nitrite Uptake by Higher Plant Chloroplasts. Plant Cell Physiol 48: 1022-1035 [Abstract] [Full Text]  
• Boczko, E. M., Cooper, T. G., Gedeon, T., Mischaikow, K., Murdock, D. G., Pratap, S., Wells, K. S. (2005). Structure theorems and the dynamics of nitrogen catabolite repression in yeast. Proc. Natl. Acad. Sci. USA 102: 5647-5652 [Abstract] [Full Text]  
• Powell, C. D., Quain, D. E., Smart, K. A. (2003). Chitin scar breaks in aged Saccharomyces cerevisiae. Microbiology 149: 3129-3137 [Abstract] [Full Text]  
• Laabs, T. L., Markwardt, D. D., Slattery, M. G., Newcomb, L. L., Stillman, D. J., Heideman, W. (2003). ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 100: 10275-10280 [Abstract] [Full Text]  
• Tan, Y. Y., Spiering, M. J., Scott, V., Lane, G. A., Christensen, M. J., Schmid, J. (2001). In Planta Regulation of Extension of an Endophytic Fungus and Maintenance of High Metabolic Rates in Its Mycelium in the Absence of Apical Extension. Appl. Environ. Microbiol. 67: 5377-5383 [Abstract] [Full Text]  
• Rabinovitch, A., Hadas, H., Einav, M., Melamed, Z., Zaritsky, A. (1999). Model for Bacteriophage T4 Development in Escherichia coli. J. Bacteriol. 181: 1677-1683 [Abstract] [Full Text]  
• Alberghina, L., Smeraldi, C., Ranzi, B. M., Porro, D. (1998). Control by Nutrients of Growth and Cell Cycle Progression in Budding Yeast, Analyzed by Double-Tag Flow Cytometry. J. Bacteriol. 180: 3864-3872 [Abstract] [Full Text]