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J Bacteriol. 1994 August; 176(16): 4890-4898

research-article

New osmoregulated beta(1-3),beta(1-6) glucosyltransferase(s) in Azospirillum brasilense.

Instituto de Investigaciones Bioquímicas Fundación Campomar, Buenos Aires, Argentina.

ABSTRACT

A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of Azospirillum brasilense cells growing in a medium of low osmotic strength. This glucan was produced in vitro by purified bacterial inner membranes with UDP-glucose as the sugar donor in the presence of Mg2+. Growth in a high-osmotic-strength medium strongly reduced the amount of this glucan accumulated in the periplasmic space, and the inhibition was associated with a reduction in the enzymatic activity of the beta(1-3),beta(1-6) glucosyltransferase(s).