This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, K J Articles by Igo, M M Search for Related Content PubMed PubMed Citation Articles by Huang, K J Articles by Igo, M M

 Previous Article  |  Next Article 

J Bacteriol. 1994 March; 176(5): 1309-1315

research-article

A distant upstream site involved in the negative regulation of the Escherichia coli ompF gene.

Section of Microbiology, University of California, Davis 95616.

ABSTRACT

The two-component regulatory system, OmpR-EnvZ, of Escherichia coli K-12 regulates the expression of the major outer membrane porin protein, OmpF. OmpR is a DNA-binding protein which acts as both an activator and a repressor to control ompF transcription. In this article, we describe a new OmpR-binding site that is located between 384 to 351 bp upstream from the ompF start point of transcription. Inactivation of this site by insertion of a 22-bp fragment prevents the repression of ompF expression conferred by the dominant negative mutation, envZ473. On the basis of the location of this binding site, the presence of bent DNA in the ompF regulatory region (T. Mizuno, Gene 54:57-64, 1987), and the fact that mutations altering integration host factor result in constitutive ompF expression (P. Tsui, V. Helu, and M. Freundlich, J. Bacteriol. 170:4950-4953, 1988), we propose that the negative regulation of ompF involves a DNA loop structure.

This article has been cited by other articles:

• Yoshida, T., Qin, L., Egger, L. A., Inouye, M. (2006). Transcription Regulation of ompF and ompC by a Single Transcription Factor, OmpR. J. Biol. Chem. 281: 17114-17123 [Abstract] [Full Text]  
• Batchelor, E., Walthers, D., Kenney, L. J., Goulian, M. (2005). The Escherichia coli CpxA-CpxR Envelope Stress Response System Regulates Expression of the Porins OmpF and OmpC. J. Bacteriol. 187: 5723-5731 [Abstract] [Full Text]  
• Flores-Valdez, M. A., Puente, J. L., Calva, E. (2003). Negative Osmoregulation of the Salmonella ompS1 Porin Gene Independently of OmpR in an hns Background. J. Bacteriol. 185: 6497-6506 [Abstract] [Full Text]  
• Walthers, D., Tran, V. K., Kenney, L. J. (2003). Interdomain Linkers of Homologous Response Regulators Determine Their Mechanism of Action. J. Bacteriol. 185: 317-324 [Abstract] [Full Text]  
• Jeong, K. J., Lee, S. Y. (2002). Excretion of Human {beta}-Endorphin into Culture Medium by Using Outer Membrane Protein F as a Fusion Partner in Recombinant Escherichia coli. Appl. Environ. Microbiol. 68: 4979-4985 [Abstract] [Full Text]  
• Boucher, P. E., Yang, M.-S., Schmidt, D. M., Stibitz, S. (2001). Genetic and Biochemical Analyses of BvgA Interaction with the Secondary Binding Region of the fha Promoter of Bordetella pertussis. J. Bacteriol. 183: 536-544 [Abstract] [Full Text]  
• Peñaloza-Vázquez, A., Bender, C. L. (1998). Characterization of CorR, a Transcriptional Activator Which Is Required for Biosynthesis of the Phytotoxin Coronatine. J. Bacteriol. 180: 6252-6259 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Painbeni, E., Caroff, M., Rouviere-Yaniv, J. (1997). Alterations of the outer membrane composition in Escherichia coli lacking the histone-like protein HU. Proc. Natl. Acad. Sci. USA 94: 6712-6717 [Abstract] [Full Text]  
• Huang, K.-J., Lan, C.-Y., Igo, M. M. (1997). Phosphorylation stimulates the cooperative DNA-binding properties of the transcription factor OmpR. Proc. Natl. Acad. Sci. USA 94: 2828-2832 [Abstract] [Full Text]  
• Dahl, J. L., Wei, B.-Y., Kadner, R. J. (1997). Protein Phosphorylation Affects Binding of the Escherichia coli Transcription Activator UhpA to the uhpT Promoter. J. Biol. Chem. 272: 1910-1919 [Abstract] [Full Text]  
• Harlocker, S. L., Bergstrom, L., Inouye, M. (1995). Tandem Binding of Six OmpR Proteins to the ompF Upstream Regulatory Sequence of Escherichia coli. J. Biol. Chem. 270: 26849-26856 [Abstract] [Full Text]  
• Jung, K., Hamann, K., Revermann, A. (2001). K+ Stimulates Specifically the Autokinase Activity of Purified and Reconstituted EnvZ of Escherichia coli. J. Biol. Chem. 276: 40896-40902 [Abstract] [Full Text]