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J. Bacteriol., Jan 1995, 259-263, Vol 177, No. 1
Copyright © 1995, American Society for Microbiology

Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes

TS Gorton, MS Goh and SJ Geary
Department of Pathobiology, University of Connecticut, Storrs 06269- 3089.

We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes.

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