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J. Bacteriol., Jan 1995, 264-267, Vol 177, No. 1
Copyright © 1995, American Society for Microbiology

The role of ribonuclease H in multicopy single-stranded DNA synthesis in retron-Ec73 and retron-Ec107 of Escherichia coli

T Shimamoto, M Shimada, M Inouye and S Inouye
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.

Bacterial reverse transcriptase is responsible for the synthesis of multicopy single-stranded DNA (msDNA). Reverse transcriptases from retron-Ec73 and retron-Ec107 do not contain an RNase H domain. Cellular RNase H is therefore considered to be required to make the mature form of msDNA. We found that RNase HI, but not RNase HII, is required for the production of the mature form of both msDNAs.


This article has been cited by other articles:

  • Singer, M. F., Singer, M. F. (1995). Unusual Reverse Transcriptases. J. Biol. Chem. 270: 24623-24626 [Full Text]  


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