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J. Bacteriol., May 1995, 2912-2913, Vol 177, No. 10
Copyright © 1995, American Society for Microbiology

Site of phosphorylation of SpoIIAA, the anti-anti-sigma factor for sporulation-specific sigma F of Bacillus subtilis

SM Najafi, AC Willis and MD Yudkin
Department of Biochemistry, University of Oxford, United Kingdom.

Sigma F is regulated by an anti-sigma factor, SpoIIAB, and an anti-anti- sigma factor, SpoIIAA. SpoIIAB also functions as a phosphokinase which transfers phosphate from ATP to SpoIIAA; this phosphorylation is thought to be involved in the regulatory mechanism. By using [gamma- 32P]ATP to phosphorylate SpoIIAA, cleaving the protein proteolytically, and analyzing the one resulting radiolabelled peptide by the Edman degradation procedure, we show that the site of phosphorylation in SpoIIAA is Ser-58.


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