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J. Bacteriol., 05 1995, 2938-2941, Vol 177, No. 10
Copyright © 1995, American Society for Microbiology

Conversion of 2-chloro-cis,cis-muconate and its metabolites 2-chloro- and 5-chloromuconolactone by chloromuconate cycloisomerases of pJP4 and pAC27

MD Vollmer and M Schlomann
Institut fur Mikrobiologie, Universitat Stuttgart, Germany.

2-Chloro-cis,cis-muconate, the product of ortho-cleavage of 3- chlorocatechol, was converted by purified preparations of the pJP4- and pAC27-encoded chloromuconate cycloisomerases (EC 5.5.1.7) to trans- dienelactone (trans-4-carboxymethylenebut-2-en-4-olide). The same compound was also formed when (+)-2-chloro- and (+)-5- chloromuconolactone were substrates of these enzyme preparations. Thus, the pJP4- and pAC27-encoded chloromuconate cycloisomerases are able to catalyze chloride elimination from (+)-5-chloromuconolactone. The ability to convert (+)-2-chloromuconolactone differentiates these enzymes from other groups of cycloisomerases.


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