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J. Bacteriol., Jun 1995, 3337-3343, Vol 177, No. 11
Copyright © 1995, American Society for Microbiology

Physical and genetic map of the chromosome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803

YN Churin, IN Shalak, T Borner and SV Shestakov
Department of Genetics, Humboldt University Berlin, Germany.

A combined physical and genetic map of the cyanobacterium Synechocystis sp. strain PCC 6803 chromosome was constructed. An estimated genome size of 3.82 Mb was obtained by summing the sizes of 25 MluI or 40 NotI fragments seen by pulsed-field electrophoresis. The order of the restriction fragments was determined by using two independent experimental approaches: pulsed-field fragment hybridization and linking clone analysis. The relative positions of 30 known genes or gene clusters were localized.

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• Singh, A. K., Sherman, L. A. (2000). Identification of Iron-Responsive, Differential Gene Expression in the Cyanobacterium Synechocystis sp. Strain PCC 6803 with a Customized Amplification Library. J. Bacteriol. 182: 3536-3543 [Abstract] [Full Text]