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J. Bacteriol., Jun 1995, 3610-3612, Vol 177, No. 12
Copyright © 1995, American Society for Microbiology

SOS induction in Escherichia coli by single-stranded DNA of mutant filamentous phage: monitoring by cleavage of LexA repressor

N Higashitani, A Higashitani and K Horiuchi
Division of Microbial Genetics, National Institute of Genetics, Mishima, Japan.

Infection of Escherichia coli in the presence of chloramphenicol with mutant filamentous phage that are defective in the initiation of minus- strand DNA synthesis induces the SOS response as monitored by cellular LexA levels. This observation demonstrates that single-stranded DNA serves as a primary signal for SOS induction in vivo.


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